The polymerase chain reaction (PCR) converts very low quantities of DNA into very high quantities and is the foundation of many specialized techniques of molecular biology. PCR utilizes components of the cellular machinery of mitotic cell division in vitro which respond predictably to user inputs. This chapter introduces the principles of PCR and discusses practical considerations from target sequence definition through to optimization and application.
Journal article
The polymerase chain reaction (PCR): general methods
Methods in Molecular Biology, Vol.1099, pp.65-75
2014
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Abstract
Details
- Title
- The polymerase chain reaction (PCR): general methods
- Creators
- Daniel LE Waters - Southern Cross University
- Publication Details
- Methods in Molecular Biology, Vol.1099, pp.65-75
- Identifiers
- 1784; 991012821023202368
- Academic Unit
- Southern Cross Plant Science
- Resource Type
- Journal article