An In Vitro Phytohormone Survey Reveals Concerted Regulation of the Cannabis Glandular Trichome Disc Cell Proteome

Nicolas Dimopoulos; Qi Guo; Lei Liu; Matthew Nolan; Rekhamani Das; Lennard Garcia-de Heer; Jos C. Mieog; Bronwyn J. Barkla; Tobias Kretzschmar

doi:10.3390/plants14050694

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An In Vitro Phytohormone Survey Reveals Concerted Regulation of the Cannabis Glandular Trichome Disc Cell Proteome

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An In Vitro Phytohormone Survey Reveals Concerted Regulation of the Cannabis Glandular Trichome Disc Cell Proteome

Nicolas Dimopoulos, Qi Guo, Lei Liu, Matthew Nolan, Rekhamani Das, Lennard Garcia-de Heer, Jos C. Mieog, Bronwyn J. Barkla and Tobias Kretzschmar

Plants (Basel), Vol.14(5), 694

24/02/2025

DOI: https://doi.org/10.3390/plants14050694

Appears in Recent Faculty of Science and Engineering Publications

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Abstract

<italic>Cannabis sativa</italic>

jasmonic acid

salicylic acid

cytokinin

diethyldithiocarbamate

cannabinoids

leucoplast

plastid

cuticular wax

metabolism

Cannabis (Cannabis sativa L.) flower glandular trichomes (GTs) are the main site of cannabinoid synthesis. Phytohormones, such as jasmonic acid (JA) and salicylic acid (SA), have been shown to increase cannabinoid content in cannabis flowers, but how this is regulated remains unknown. This study aimed to understand which biological processes in GT disc cells phytohormones control by using an in vitro assay. Live GT disc cells were isolated from a high-tetrahydrocannabinol cannabis cultivar and incubated on basal media plates supplemented with either kinetin (KIN), JA, SA, abscisic acid, ethephon, gibberellic acid, brassinolide, or sodium diethyldithiocarbamate. Quantitative proteomic analysis revealed that KIN, JA, and SA caused the greatest number of changes in the GT disc cell proteome. Surprisingly, none of the treatments concertedly increased cannabinoid content or the abundance of related biosynthetic proteins in the GT, suggesting that cannabinoid increases in previous in planta phytohormone studies are likely due to other processes, such as increased GT density. As well, KIN-, JA-, and SA-treated GTs had numerous differentially abundant proteins in common. Several were key proteins for leucoplast differentiation, cuticular wax and fatty acid metabolism, and primary metabolism regulation, denoting that cytokinin, JA, and SA signalling are likely important for coordinating cannabis GT differentiation and development.

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Title: An In Vitro Phytohormone Survey Reveals Concerted Regulation of the Cannabis Glandular Trichome Disc Cell Proteome
Creators: Nicolas Dimopoulos - Southern Cross University
Qi Guo - Southern Cross University
Lei Liu - Southern Cross University
Matthew Nolan - Southern Cross University
Rekhamani Das - Southern Cross University
Lennard Garcia-de Heer
Jos C. Mieog - Southern Cross University
Bronwyn J. Barkla - Southern Cross University
Tobias Kretzschmar - Southern Cross University
Publication Details: Plants (Basel), Vol.14(5), 694
Publisher: MDPI
Grant note: This project received grant funding from the Australian Government via the Department of Industry, Science, Energy and Resources, as part of the Cooperative Research Centres Project (CRC-P) program—Round 7—Growing the medicinal Cannabis industry—precision farming to pharmaceuticals. The CRC-P program is an industry-led research collaboration and Cann Group Ltd. is the lead partner organisation.
Identifiers: 991013264909602368
Academic Unit: Faculty of Science and Engineering; Research Infrastructure and Operations; Science
Language: English
Resource Type: Journal article

An In Vitro Phytohormone Survey Reveals Concerted Regulation of the Cannabis Glandular Trichome Disc Cell Proteome

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