Journal article
An In Vitro Phytohormone Survey Reveals Concerted Regulation of the Cannabis Glandular Trichome Disc Cell Proteome
Plants (Basel), Vol.14(5), 694
24/02/2025
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Abstract
Cannabis (Cannabis sativa L.) flower glandular trichomes (GTs) are the main site of cannabinoid synthesis. Phytohormones, such as jasmonic acid (JA) and salicylic acid (SA), have been shown to increase cannabinoid content in cannabis flowers, but how this is regulated remains unknown. This study aimed to understand which biological processes in GT disc cells phytohormones control by using an in vitro assay. Live GT disc cells were isolated from a high-tetrahydrocannabinol cannabis cultivar and incubated on basal media plates supplemented with either kinetin (KIN), JA, SA, abscisic acid, ethephon, gibberellic acid, brassinolide, or sodium diethyldithiocarbamate. Quantitative proteomic analysis revealed that KIN, JA, and SA caused the greatest number of changes in the GT disc cell proteome. Surprisingly, none of the treatments concertedly increased cannabinoid content or the abundance of related biosynthetic proteins in the GT, suggesting that cannabinoid increases in previous in planta phytohormone studies are likely due to other processes, such as increased GT density. As well, KIN-, JA-, and SA-treated GTs had numerous differentially abundant proteins in common. Several were key proteins for leucoplast differentiation, cuticular wax and fatty acid metabolism, and primary metabolism regulation, denoting that cytokinin, JA, and SA signalling are likely important for coordinating cannabis GT differentiation and development.
Details
- Title
- An In Vitro Phytohormone Survey Reveals Concerted Regulation of the Cannabis Glandular Trichome Disc Cell Proteome
- Creators
- Nicolas Dimopoulos - Southern Cross UniversityQi Guo - Southern Cross UniversityLei Liu - Southern Cross UniversityMatthew Nolan - Southern Cross UniversityRekhamani Das - Southern Cross UniversityLennard Garcia-de HeerJos C. Mieog - Southern Cross UniversityBronwyn J. Barkla - Southern Cross UniversityTobias Kretzschmar - Southern Cross University
- Publication Details
- Plants (Basel), Vol.14(5), 694
- Publisher
- MDPI
- Grant note
- This project received grant funding from the Australian Government via the Department of Industry, Science, Energy and Resources, as part of the Cooperative Research Centres Project (CRC-P) program—Round 7—Growing the medicinal Cannabis industry—precision farming to pharmaceuticals. The CRC-P program is an industry-led research collaboration and Cann Group Ltd. is the lead partner organisation.
- Identifiers
- 991013264909602368
- Academic Unit
- Research Infrastructure and Operations; Faculty of Science and Engineering; Science
- Language
- English
- Resource Type
- Journal article