Files and links (3)
READMECC BY V4.0, Open Access
Supplementary MaterialCC BY V4.0, Open Access
CC BY V4.0, Open
Dataset
An in vitro phytohormone survey reveals concerted regulation of the Cannabis glandular trichome proteome
Southern Cross University
19/12/2024
DOI:
https://doi.org/10.25918/data.382
Metrics
12 File views/ downloads
100 Record Views
Abstract
<p>Glandular trichomes (GTs) are major sites of cannabinoid and terpenoid metabolism in <em>Cannabis sativa</em> L flowers. Several phytohormones, such as jasmonic acid (JAS) and salicylic acid (SAL) cause increased cannabinoid content in <em>Cannabis</em> flowers, but how they regulate GTs to cause this change is unknown. This study aimed to understand the mechanisms of action of phytohormones on mature GTs using a novel <em>in vitro</em> assay. Live GTs were isolated from ‘Hindu Kush’ <em >Cannabis</em>, a high-tetrahydrocannabinol cultivar, and incubated on basal media plates with either kinetin (KIN), JAS, SAL, abscisic acid, ethephon, gibberellic acid, brassinolide, or sodium diethyldithiocarbamate (DIE), an inhibitor of jasmonic acid synthesis. Quantitative proteomic analysis (SWATH-MS) revealed that KIN, JAS and SAL caused the greatest number of changes in the GT proteome while the other treatments exerted much smaller changes. Surprisingly, metabolite analysis showed none of the treatments caused cannabinoids to increase in the GT, while DIE caused cannabigerolic acid content to decrease. Nor was there any concerted increased abundance of cannabinoid biosynthetic pathway proteins by any treatments. This indicates that cannabinoid increases in previous <em>in planta</em> phytohormone studies are likely due to other processes, such as through increased GT density. As well, KIN, JAS and SAL treated GT’s had a large proportion of differentially abundant proteins in common. Several were key proteins for leucoplast differentiation and function, cuticular wax and fatty acid metabolism and primary metabolism regulation. This points towards cytokinin, jasmonic acid and salicylic acid signalling likely being important for coordinating <em>Cannabis</em> GT differentiation and development. </p>
Details
- Title
- An in vitro phytohormone survey reveals concerted regulation of the Cannabis glandular trichome proteome
- Creators
- Nicolas Dimopoulos (Author) - Southern Cross UniversityQi Guo (Author) - Southern Cross University, Southern Cross Analytical Research ServicesBen Liu (Contributor) - Southern Cross University, ScienceMatt Nolan (Contributor)Rekhamani Das (Contributor) - Southern Cross UniversityLennard Garcia-de Heer (Contributor) - Southern Cross University, Faculty of Science and EngineeringJos C Mieog (Contributor) - Southern Cross University, ScienceBronwyn J Barkla (Contributor) - Southern Cross University, ScienceTobias Kretzschmar (Contributor) - Southern Cross University, Science
- Publisher
- Southern Cross University
- Identifiers
- 991013205013802368
- Academic Unit
- Faculty of Science and Engineering
- Language
- English
- Resource Type
- Dataset