Files and links (2)
Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
Variation in seed longevity among diverse Indica rice varieties
Annals of Botany, Vol.124(3), pp.447-460
16/08/2019
PMCID: PMC6798842
PMID: 31180503
Metrics
7 File views/ downloads
27 Record Views
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Abstract
Understanding variation in seed longevity, especially within closely related germplasm, will lead to better understanding of the molecular basis of this trait, which is particularly important for seed genebanks, but is also relevant to anyone handling seeds. We therefore set out to determine the relative seed longevity of diverse Indica rice accessions through storage experiments. Since antioxidants are purported to play a role in seed storability, the antioxidant activity and phenolic content of caryopses were determined.
Seeds of 299 Indica rice accessions harvested at 31, 38 and 45 d after heading (DAH) between March and May 2015 and differing in harvest moisture content (MC) were subsequently stored at 10.9 % MC and 45 °C. Samples were taken at regular intervals and sown for germination. Germination data were subjected to probit analysis and the resulting parameters that describe the loss of viability during storage were used for genome-wide association (GWA) analysis.
The seed longevity parameters, Ki [initial viability in normal equivalent deviates (NED)], -σ-1 (σ is the time for viability to fall by 1 NED in experimental storage) and p50 [time for viability to fall to 50 % (0 NED)], varied considerably across the 299 Indica accessions. Seed longevity tended to increase as harvest MC decreased and to decrease as harvest MC increased. Eight major loci associated with seed longevity parameters were identified through GWA analysis. The favourable haplotypes on chromosomes 1, 3, 4, 9 and 11 enhanced p50 by ratios of 0.22-1.86.
This is the first study to describe the extent of variation in σ within a species' variety group. A priori candidate genes selected based on rice genome annotation and gene network ontology databases suggested that the mechanisms conferring high seed longevity might be related to DNA repair and transcription, sugar metabolism, reactive oxygen species scavenging and embryonic/root development.
Details
- Title
- Variation in seed longevity among diverse Indica rice varieties
- Creators
- Jae-Sung Lee - International Rice Research InstituteMarlina Velasco-Punzalan - International Rice Research InstituteMyrish Pacleb - International Rice Research InstituteRocel Valdez - International Rice Research InstituteTobias Kretzschmar - International Rice Research InstituteKenneth L McNally - International Rice Research InstituteAbdel M Ismail - International Rice Research InstitutePompe C Sta Cruz - University of the Philippines Los BañosN Ruaraidh Sackville Hamilton - International Rice Research InstituteFiona R Hay - International Rice Research Institute
- Publication Details
- Annals of Botany, Vol.124(3), pp.447-460
- Publisher
- Oxford University Press; England
- Grant note
- This research was funded through the CGIAR Research Program for Managing and Sustaining Crop Collections.
- Identifiers
- 991012925578402368
- Copyright
- © The Author(s) 2019. Published by Oxford University Press on behalf of the Annals of Botany Company. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Academic Unit
- School of Environment, Science and Engineering; Southern Cross Plant Science; Faculty of Science and Engineering; Science
- Language
- English
- Resource Type
- Journal article