Journal article
Trends in molluscan gene sequence similarity: an observation from genes expressed within the hypobranchial gland of Dicathais orbita (Gmelin, 1791) (Neogastropoda: Muricidae)
The Nautilus, Vol.123(3), pp.154-158
2009
Metrics
24 Record Views
Abstract
This study investigates the phylogenetic distribution of homology to Dicathais orbita hypobranchial gland genes based on tBLASTx pairwise sequence alignments from the Genbank database. Suppressive subtractive hybridization was used to obtain 417 non-redundant genes that were up-regulated or uniquely expressed in the hypobranchial gland relative to mantle tissue. Of these, 133 sequences revealed matches to the database with the remaining 68% of genes appearing as apparently novel sequences. Homologous sequence matches were observed for a wide range of evolutionarily divergent taxa, encompassing animals, protozoans, plants, fungi, bacteria and viruses. The highest frequency of homology was found towards chordate sequences, followed by the Mollusca, which high-lights the current bias in availability of vertebrate versus invertebrate sequences in the database. An unexpectedly high proportion of matches were also found toward the Ciliophora, indicating a possible symbiotic relationship, as well as the Ascomycota and Streptophyta, which share as Dicathais orbita. Overall, these results reveal the usefulness of undertaking sequence comparisons in gene expression and highlight the current paucity of knowledge of molluscan genomes.
Details
- Title
- Trends in molluscan gene sequence similarity: an observation from genes expressed within the hypobranchial gland of Dicathais orbita (Gmelin, 1791) (Neogastropoda: Muricidae)
- Creators
- Patrick W LaffyKirsten Benkendorff - Southern Cross UniversityCatherine A Abbott
- Publication Details
- The Nautilus, Vol.123(3), pp.154-158
- Number of pages
- 154-158
- Identifiers
- 4606; 991012821099002368
- Academic Unit
- Marine Ecology Research Centre; School of Environment, Science and Engineering; Faculty of Science and Engineering; Science
- Resource Type
- Journal article