Journal article
Transposon insertions within alleles of BnaFT.A2 are associated with seasonal crop type in rapeseed
Theoretical and Applied Genetics, Vol.135(10), pp.3469-3483
10/2022
PMID: 35997786
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Source: InCites
Abstract
Florigen, encoded by FLOWERING LOCUS T (FT), plays key roles not only as a flowering hormone, but also a universal growth factor affecting several aspects of plant architecture. In rapeseed, BnaFT.A2 has been revealed as one of the major loci associated with flowering time and different ecotypes. However, it is unclear how allelic variations of BnaFT.A2 affect its function in flowering time regulation and beyond. In this study, we confirmed an existing 288 bp miniature inverted-repeat transposable element (MITE) insertion within the second intron and identified two new insertions within the promoter of BnaFT.A2—a 3971 bp CACTA and a 1079 bp Helitron. Each insertion event corresponds to a distinct BnaFT.A2 haplotype and is closely associated with established crop seasonal ecotypes. These alleles have similar tissue-specific expression patterns but discrete transcriptional patterns tightly associated with rapeseed flowering time and ecotype. RNAi lines and mutants of BnaFT.A2 flowered significantly later than controls. Differentially expressed genes (DEGs), identified in transcriptomic profiling of seedling leaves from two loss-of-function mutants (Bnaft.a2-L1 and Bnaft.a2-L2) compared with controls, indicated significant enrichment for hormone metabolic genes and roles related to plant cell wall synthesis and photosynthesis. Plants with loss-of-function BnaFT.A2 had smaller leaves and lower net photosynthetic rate compared to controls. These findings not only further clarify the genetic basis of flowering time variation and ecotype formation in B. napus, but also provide an additional toolbox for genetic improvement of seasonal adaptation and production.
Details
- Title
- Transposon insertions within alleles of BnaFT.A2 are associated with seasonal crop type in rapeseed
- Creators
- Qingdong Jin - Huazhong Agricultural UniversityGengdong Gao - Huazhong Agricultural UniversityChaocheng Guo - Huazhong Agricultural UniversityTaihua Yang - Huazhong Agricultural UniversityGe Li - Huazhong Agricultural UniversityJurong Song - Huazhong Agricultural UniversityNa Zheng - Huazhong Agricultural UniversityShuai Yin - Huazhong Agricultural UniversityLicong Yi - Hubei Academy of Agricultural SciencesZhen Li - Jinhua PolytechnicXianhong Ge - Huazhong Agricultural UniversityGraham J. King - Southern Cross UniversityJing Wang - Huazhong Agricultural UniversityGuangsheng Zhou - Huazhong Agricultural University
- Publication Details
- Theoretical and Applied Genetics, Vol.135(10), pp.3469-3483
- Publisher
- Springer
- Grant note
- No. LQ18C060004 / Natural Science Foundation of Hunan Province (http://dx.doi.org/10.13039/501100004735) 2018YFD1000900 / Key Technologies Research and Development Program (http://dx.doi.org/10.13039/501100012165)
- Identifiers
- 991013052393302368
- Copyright
- © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022
- Academic Unit
- Southern Cross Plant Science; Faculty of Science and Engineering; Science
- Language
- English
- Resource Type
- Journal article