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Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples
Journal article   Peer reviewed

Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples

Xiaole Yin, Xi Chen, Xiao-Tao Jiang, Ying Yang, Bing Li, Marcus Ho-Hin Shum, Tommy T. Y. Lam, Gabriel M. Leung, Joan Rose, Concepcion Sanchez-Cid, …
Environmental Science & Technology, Vol.57(26), pp.9713-9721
13/06/2023
PMID: 37310875

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Source: InCites

Abstract

one-health metagenomics long-read sequencing ARG copy per cell standardization
Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys in human and animal sectors under the “One-Health” framework. However, there are substantial challenges in comparing and synthesizing the results of multiple studies that employ different test methods and approaches in bioinformatic analysis. In this article, we consider the commonly used quantification units (ARG copy per cell, ARG copy per genome, ARG density, ARG copy per 16S rRNA gene, RPKM, coverage, PPM, etc.) for profiling ARGs and suggest a universal unit (ARG copy per cell) for reporting such biological measurements of samples and improving the comparability of different surveillance efforts.

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