Journal article
The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses
Plant Physiology (Online), Vol.190(4), pp.2617-2636
12/2022
Metrics
32 Record Views
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Abstract
A plant’s oxygen supply can vary from normal (normoxia) to total depletion (anoxia). Tolerance to anoxia is relevant to wetland species, rice (Oryza sativa) cultivation, and submergence tolerance of crops. Decoding and transmitting calcium (Ca) signals may be an important component to anoxia tolerance; however, the contribution of intracellular Ca transporters to this process is poorly understood. Four functional cation/proton exchangers (CAX1–4) in Arabidopsis (Arabidopsis thaliana) help regulate Ca homeostasis around the vacuole. Our results demonstrate that cax1 mutants are more tolerant to both anoxic conditions and submergence. Using phenotypic measurements, RNA-sequencing, and proteomic approaches, we identified cax1-mediated anoxia changes that phenocopy changes present in anoxia-tolerant crops: altered metabolic processes, diminished reactive oxygen species production post anoxia, and altered hormone signaling. Comparing wild-type and cax1 expressing genetically encoded Ca indicators demonstrated altered cytosolic Ca signals in cax1 during reoxygenation. Anoxia-induced Ca signals around the plant vacuole are involved in the control of numerous signaling events related to adaptation to low oxygen stress. This work suggests that cax1 anoxia response pathway could be engineered to circumvent the adverse effects of flooding that impair production agriculture.
Details
- Title
- The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses
- Creators
- Jian Yang - Baylor College of MedicineIny Elizebeth Mathew - Baylor College of MedicineHormat Rhein - Baylor College of MedicineRichard Barker - University of Wisconsin–MadisonQi Guo - Southern Cross UniversityLuca BrunelloElena LoretiBronwyn J Barkla - Southern Cross UniversitySimon GilroyPierdomenico PerataKendal D Hirschi - Baylor College of Medicine
- Publication Details
- Plant Physiology (Online), Vol.190(4), pp.2617-2636
- Publisher
- American Society of Plant Biologists
- Grant note
- This work was supported by grants (to K.D.H) from the National Science Foundation (1557890), USDA (3092-51000-061-00D), and National Institute of Health (R03 AI149201-02) and grants (to S.G) from NSF MCB2016177 and NASA 80NSSC19K0126.
- Identifiers
- 991013054811302368
- Copyright
- © American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com
- Academic Unit
- Faculty of Science and Engineering; Science; Southern Cross Plant Science
- Language
- English
- Resource Type
- Journal article