The rooting ability of in vitro shoot cultures established from a UK collection of the common ash (Fraxinus excelsior L.) and their ex vitro survival

Trevor Fenning; Margaret O’Donnell; Katharine Preedy; Aurélia Bézanger; David Kenyon; Gustavo Lopez

doi:10.1186/s13595-022-01146-8

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The rooting ability of in vitro shoot cultures established from a UK collection of the common ash (Fraxinus excelsior L.) and their ex vitro survival

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The rooting ability of in vitro shoot cultures established from a UK collection of the common ash (Fraxinus excelsior L.) and their ex vitro survival

Trevor Fenning, Margaret O’Donnell, Katharine Preedy, Aurélia Bézanger, David Kenyon and Gustavo Lopez

Annals of forest science, Vol.79, pp.1-16

23/06/2022

DOI: https://doi.org/10.1186/s13595-022-01146-8

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Abstract

Biomedical and Life Sciences

Environment

Forestry

Forestry Management

general

Life Sciences

Research Paper

Tree Biology

Wood Science & Technology

European ash

Ash dieback

Chalara fraxinea

Hymenoscyphus fraxineus

In vitro propagation

Rooting

Bacillus megaterium

Aims This work aimed to develop and test a set of standard tissue culture methods for propagating material from selected ash trees, and then to produce rooted clonal plants which could be transferred to nursery conditions. Methods Seed material from a range of British and Irish ash provenances were used for this work, with the shoot cultures being maintained on DKW based media, with 3 ppm BAP being added to induce shoot proliferation, with the shoots so generated being exposed to 3 ppm IBA to induce root formation in vitro. Results One hundred thirty-six shoot cultures were successfully established from 13 mother trees. Most were generated from hypocotyl pieces excised from sterile germinating ash seeds on DKW medium plus 3 ppm BAP. Another 24 cultures were lost to a bacterial contaminant, which was provisionally identified as the plant symbiont Bacillus megaterium or a close relative. Overall, 41.5% of uncontaminated plants and 11.6% of contaminated plants produced roots in vitro, after exposure to DKW medium with 3 ppm IBA, followed by hormone-free medium, with 92.6% of the uncontaminated plants surviving the transfer to the nursery whether they were rooted or not, as opposed to 62.1% of the contaminated plants. Conclusions This methodology can be used to produce large numbers of clonal ash plants on demand from a wide cross-section of the UK’s and Ireland’s ash breeding population, and so can be used to help produce the ash plants needed to combat ash die-back disease, although contamination issues are likely to remain an issue.

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Title: The rooting ability of in vitro shoot cultures established from a UK collection of the common ash (Fraxinus excelsior L.) and their ex vitro survival
Creators: Trevor Fenning - Southern Cross University
Margaret O’Donnell - Forest Research, Northern Research Station
Katharine Preedy - Biomathematics and Statistics Scotland
Aurélia Bézanger - James Hutton Institute
David Kenyon - Diagnostics, Wildlife & Molecular Biology Unit, SASA
Gustavo Lopez - Forest Research, Northern Research Station
Publication Details: Annals of forest science, Vol.79, pp.1-16
Publisher: Springer Paris
Grant note: Living Ash Project grant TH0133 / Department for Environment, Food and Rural Affairs (http://dx.doi.org/10.13039/501100000277)
Identifiers: 991013035772402368
Copyright: © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Academic Unit: Faculty of Science and Engineering
Language: English
Resource Type: Journal article

The rooting ability of in vitro shoot cultures established from a UK collection of the common ash (Fraxinus excelsior L.) and their ex vitro survival

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