The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing

J Travis; M Malone; H Hu; A Baten; K Johani; F Huygens; K Vickery; K Benkendorff

doi:10.1186/s12866-020-01843-2

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The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing

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The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing

J Travis, M Malone, H Hu, A Baten, K Johani, F Huygens, K Vickery and K Benkendorff

BMC microbiology, Vol.20(1), pp.1-163

16/06/2020

DOI: https://doi.org/10.1186/s12866-020-01843-2

PMID: 32546123

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Abstract

Wound pathogens

16S rRNA gene sequencing

Wound sampling methods

Diabetic foot ulcers

Swabs

Tissue biopsies

Details

Title: The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing
Creators: J Travis (Author) - Southern Cross University
M Malone - Limb Preservation and Wound Research Academic Unit, Western Sydney LHD, Liverpool, Sydney, NSW 2170 Australia
H Hu (Author) - Macquarie University
A Baten (Author) - AgResearch
K Johani (Author) - Ingham Institute
F Huygens (Author) - Queensland University of Technology
K Vickery (Author) - Macquarie University
K Benkendorff (Author) - Southern Cross University
Publication Details: BMC microbiology, Vol.20(1), pp.1-163
Publisher: BioMed Central; London
Grant note: SP41-1 / ; This research was supported by a PhD scholarship and small research grant from the Wound Management and Innovation Co-operative Research Centre (WMICRC). Collection of DFU samples and sequencing of tissue samples was supported by an Early Career Research Grant from South West Sydney Local Health District.
Identifiers: 991012925424202368
Academic Unit: Faculty of Science and Engineering; National Marine Science Centre; Science
Language: English
Resource Type: Journal article

The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing

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