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Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
The genome of the endangered Macadamia jansenii displays little diversity but represents an important genetic resource for plant breeding
Plant Direct, Vol.5(12), pp.1-11
14/12/2021
PMCID: PMC8671617
PMID: 34938939
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Source: InCites
Abstract
Macadamia, a recently domesticated expanding nut crop in the tropical and subtropical regions of the world, is one of the most economically important genera in the diverse and widely adapted Proteaceae family. All four species of Macadamia are rare in the wild with the most recently discovered, M. jansenii, being endangered. The M. jansenii genome has been used as a model for testing sequencing methods using a wide range of long read sequencing techniques. Here, we report a chromosome level genome assembly, generated using a combination of Pacific Biosciences sequencing and Hi-C, comprising 14 pseudo-molecules, with a N50 of 52 Mb and a total genome assembly size of 758 Mb of which 56% is repetitive. Completeness assessment revealed that the assembly covered −97.1% of the conserved single copy genes. Annotation predicted 31,591 protein coding genes and allowed the characterization of genes encoding biosynthesis of cyanogenic glycosides, fatty acid metabolism, and anti-microbial proteins. Re-sequencing of seven other genotypes confirmed low diversity and low heterozygosity within this endangered species. Important morphological characteristics of this species such as small tree size and high kernel recovery suggest that M. jansenii is an important source of these commercial traits for breeding. As a member of a small group of families that are sister to the core eudicots, this high-quality genome also provides a key resource for evolutionary and comparative genomics studies.
Details
- Title
- The genome of the endangered Macadamia jansenii displays little diversity but represents an important genetic resource for plant breeding
- Creators
- Priyanka Sharma - University of QueenslandValentine Murigneux - University of QueenslandJasmine Haimovitz - Dovetail Genomics (United States)Catherine J Nock - Southern Cross UniversityWei Tian - BGI ShenzhenArdashir Kharabian Masouleh - University of QueenslandBruce Topp - University of QueenslandMobashwer Alam - University of QueenslandAgnelo Furtado - University of QueenslandRobert J Henry - University of Queensland
- Publication Details
- Plant Direct, Vol.5(12), pp.1-11
- Publisher
- John Wiley & Sons, Inc.
- Number of pages
- 11
- Grant note
- University of Queensland Hort Innovation
- Identifiers
- 991012987892802368
- Copyright
- This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2021 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.
- Academic Unit
- Faculty of Science and Engineering; Science; Southern Cross Plant Science
- Language
- English
- Resource Type
- Journal article