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The effect of in vitro digestion on the anti-allergic, anti-inflammatory and antioxidant properties1.57 MB
Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
The effect of in vitro digestion on the anti-allergic, anti-inflammatory and antioxidant properties of purple rice and purple barley phenolic extracts in Caco-2 and RBL-2H3 cells
Food bioscience, Vol.61, 104943
10/2024
Appears in Recent Faculty of Health Publications
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Abstract
Gastrointestinal diseases are associated with increased oxidative stress and inflammation. Pigmented cereal polyphenols are believed to have therapeutic potential. However, the impact of digestion on their bioactivity has not been fully elucidated. This study investigated the impact of digestion on the ability of purple rice extract (PRx) and purple barley (PBx) to alleviate oxidative stress-induced intestinal epithelial cell death and allergic inflammation. The study used a human colon adenocarcinoma (Caco-2) cell line to simulate intestinal oxidative stress for 4 h using 1 mM hydrogen peroxide. To evaluate the protective effects of digested and undigested cereal extracts, cells were first pre-treated with varying concentrations (50, 100, 200, and 500 μg/ml) of both purple rice (PRx) and purple barley (PBx) extracts. A rat basophilic leukemia (RBL-2H3) cell line was used to investigate the ability of the extracts to prevent calcium ionophore-induced histamine and cytokine release. The results demonstrated that both digested and undigested phenolic extracts prevented oxidative stress-induced cell death in Caco-2 cells and reduced Interleukin (IL)-8 secretion. PRx showed greater cytoprotective effects than PBx. Both digested and undigested extracts showed a time-dependent antihistamine effect, with the greatest inhibitory effects observed after 2 h and 4 h of treatment with PRx. Additionally, the extracts significantly attenuated the release of several cytokines including IL-2, IL-4, IL-13, monocyte chemoattractant protein-1, interferon-gamma, and tumour necrosis factor-alpha. Overall, the study demonstrates that both digested and undigested pigmented rice and barley extracts are potential sources of polyphenols which may promote intestinal health and alleviate allergic inflammation.
Details
- Title
- The effect of in vitro digestion on the anti-allergic, anti-inflammatory and antioxidant properties of purple rice and purple barley phenolic extracts in Caco-2 and RBL-2H3 cells
- Creators
- Borkwei Ed Nignpense - Charles Sturt UniversityBoris Budiono - Charles Sturt UniversityNidhish Francis - Charles Sturt UniversityChristopher L. Blanchard - Charles Sturt UniversityAbishek B. Santhakumar - Charles Sturt University
- Publication Details
- Food bioscience, Vol.61, 104943
- Publisher
- Elsevier Ltd
- Grant note
- The authors would like to thank Thermofisher support team for their invaluable technical assistance. The authors would also like to acknowledge the Gulbali Institute Bridging Scheme for providing financial support to the first author.
- Identifiers
- 991013215113602368
- Copyright
- © 2024 The Authors.
- Academic Unit
- Faculty of Health
- Language
- English
- Resource Type
- Journal article