Journal article
Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice
Plant and Cell Physiology, Vol.57(5), pp.919-932
05/2016
PMID: 26903527
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Source: InCites
Abstract
The glycine decarboxylase complex (GDC) plays a critical role in the photorespiratory C2 cycle of C3 species by recovering carbon following the oxygenation reaction of ribulose-1,5-bisphosphate carboxylase/oxygenase. Loss of GDC from mesophyll cells (MCs) is considered a key early step in the evolution of C4 photosynthesis. To assess the impact of preferentially reducing GDC in rice MCs, we decreased the abundance of OsGDCH (Os10g37180) using an artificial microRNA (amiRNA) driven by a promoter that preferentially drives expression in MCs. GDC H- and P-proteins were undetectable in leaves of gdch lines. Plants exhibited a photorespiratory-deficient phenotype with stunted growth, accelerated leaf senescence, reduced chlorophyll, soluble protein and sugars, and increased glycine accumulation in leaves. Gas exchange measurements indicated an impaired ability to regenerate ribulose 1,5-bisphosphate in photorespiratory conditions. In addition, MCs of gdch lines exhibited a significant reduction in chloroplast area and coverage of the cell wall when grown in air, traits that occur during the later stages of C4 evolution. The presence of these two traits important for C4 photosynthesis and the non-lethal, down-regulation of the photorespiratory C2 cycle positively contribute to efforts to produce a C4 rice prototype.
Details
- Title
- Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C4 Rice
- Creators
- HsiangChun Lin - C4 Rice Center, International Rice Research Institute, Los Baños, Philippines These authors contributed equally to this workShanta Karki - C4 Rice Center, International Rice Research Institute, Los Baños, Philippines These authors contributed equally to this workRobert A Coe - C4 Rice Center, International Rice Research Institute, Los Baños, Philippines These authors contributed equally to this workShaheen Bagha - Department of Ecology & Evolutionary Biology, University of Toronto, Toronto, M5S 3B2, CanadaRoxana Khoshravesh - Department of Ecology & Evolutionary Biology, University of Toronto, Toronto, M5S 3B2, CanadaC Paolo Balahadia - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesJulius Ver Sagun - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesRonald Tapia - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesW Krystler Israel - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesFlorencia Montecillo - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesAlbert de Luna - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesFlorence R Danila - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesAndrea Lazaro - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesCzarina M Realubit - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesMichelle G Acoba - C4 Rice Center, International Rice Research Institute, Los Baños, PhilippinesTammy L Sage - Department of Ecology & Evolutionary Biology, University of Toronto, Toronto, M5S 3B2, CanadaSusanne von Caemmerer - ARC Centre of Excellence for Translational Photosynthesis, Research School of Biology, The Australian National University, Acton, 2601, AustraliaRobert T Furbank - ARC Centre of Excellence for Translational Photosynthesis, Research School of Biology, The Australian National University, Acton, 2601, AustraliaAsaph B Cousins - School of Biological Sciences, Molecular Plant Sciences, Washington State University, Pullman, WA 99164-4236, USAJulian M Hibberd - Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UKW Paul Quick - C4 Rice Center, International Rice Research Institute, Los Baños, Philippines Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UKSarah Covshoff - Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK sc603@cam.ac.uk
- Publication Details
- Plant and Cell Physiology, Vol.57(5), pp.919-932
- Publisher
- Oxford University Press
- Identifiers
- 991012951092602368
- Academic Unit
- Faculty of Science and Engineering; Southern Cross Plant Science
- Language
- English
- Resource Type
- Journal article