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Strong genetic differentiation and low genetic diversity in a habitat-forming fucoid seaweed (Cystophora racemosa) across 850 km of its range
Journal article   Open access   Peer reviewed

Strong genetic differentiation and low genetic diversity in a habitat-forming fucoid seaweed (Cystophora racemosa) across 850 km of its range

Jane M Edgeloe, Samuel Starko, Albert Pessarrodona, Melinda A Coleman, Jacqueline Batley, Thomas Wernberg and Georgina V Wood
Journal of phycology, Vol.61(3), pp.539-557
06/2025
PMID: 40318169
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Abstract

DArTseq Western Australia natural selection population structure macroalgae temperature genomics
Temperate seaweed forests are among the most productive and widespread habitats in coastal waters. However, they are under threat from climate change and other anthropogenic stressors. To effectively conserve and manage these ecosystems under these rising pressures, an understanding of the genetic diversity and structure of habitat-forming seaweeds will be necessary. Australia's Great Southern Reef, a global hotspot of endemic diversity, is home to one of the world's most speciose habitat-forming seaweed genera, Cystophora (order Fucales). Despite severe declines in some species, genomic data on this genus remain limited. We used a reduced representation genomic approach (DaRTSeq) to investigate the genetic diversity and structure of Cystophora racemosa, a dominant canopy-forming species, across ~850 km of its range. Our sequencing captured 4741 high-quality single nucleotide polymorphisms (SNPs), and we distinguished neutral loci from those under natural selection (i.e., outlier loci). We identified strong population structure and high genetic differentiation for both neutral (mean FST = 0.404) and outlier loci (mean FFST = 0.901). Across populations, genetic diversity was low (neutral: mean HE = 0.046; outlier: HE = 0.042), with high inferred inbreeding (neutral loci mean FIS = 0.531) and no evidence of isolation-by-distance. Several SNPs (n = 70) were observed to be putatively adaptive, with most (97%) correlated with annual maximum sea surface temperature (SST, °C), indicating local adaptation to this key ocean variable. Our results show that C. racemosa populations have low genetic diversity and high differentiation, both of which may increase the vulnerability of this important foundation species to global change.

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