Journal article
Standard addition with internal standardisation as an alternative to using stable isotope labelled internal standards to correct for matrix effects-Comparison and validation using liquid chromatography-tandem mass spectrometric assay of vitamin D
Journal of chromatography. A, Vol.1553, pp.101-107
2018
PMID: 29680744
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Source: InCites
Abstract
With mass spectrometric detection in liquid chromatography, co-eluting impurities affect the analyte response due to ion suppression/enhancement. Internal standard calibration method, using co-eluting stable isotope labelled analogue of each analyte as the internal standard, is the most appropriate technique available to correct for these matrix effects. However, this technique is not without drawbacks, proved to be expensive because separate internal standard for each analyte is required, and the labelled compounds are expensive or require synthesising. Traditionally, standard addition method has been used to overcome the matrix effects in atomic spectroscopy and was a well-established method. This paper proposes the same for mass spectrometric detection, and demonstrates that the results are comparable to those with the internal standard method using labelled analogues, for vitamin D assay. As conventional standard addition procedure does not address procedural errors, we propose the inclusion of an additional internal standard (not co-eluting). Recoveries determined on human serum samples show that the proposed method of standard addition yields more accurate results than the internal standardisation using stable isotope labelled analogues. The precision of the proposed method of standard addition is superior to the conventional standard addition method.
Details
- Title
- Standard addition with internal standardisation as an alternative to using stable isotope labelled internal standards to correct for matrix effects-Comparison and validation using liquid chromatography-tandem mass spectrometric assay of vitamin D
- Creators
- Amitha K Hewavitharana (Corresponding Author) - University of QueenslandNur Sofiah Abu Kassim - University of QueenslandPaul Nicholas Shaw - University of Queensland
- Publication Details
- Journal of chromatography. A, Vol.1553, pp.101-107
- Publisher
- Netherlands
- Identifiers
- 991012902100302368
- Academic Unit
- Southern Cross Plant Science; Science; Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article