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Stable isotopes of nitrate (δ15N and δ18O) as functional indicators of nitrogen processing in constructed wetlands
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Journal article
Stable isotopes of nitrate (δ15N and δ18O) as functional indicators of nitrogen processing in constructed wetlands
Science of The Total Environment, Vol.899, 165246
15/11/2023
PMID: 37419364
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Abstract
The effectiveness of these wetlands in removing nitrogen relies heavily on the biological processes that control its removal. Here, we used δ15N and δ18O of nitrate (NO3−) to assess the presence and the dominance of transformation processes of nitrogen in two urban water treatment wetlands in Victoria, Australia over two rainfall events. Laboratory incubation experiments were undertaken in both light and dark to measure the isotopic fractionation factor of nitrogen assimilation (by periphyton and algae) and benthic denitrification (using bare sediment). Highest isotopic fractionations were observed for nitrogen assimilation by algae and periphyton in the light, 15ε = −14.6 to −25 ‰ while the 15ε = −1.5 ‰ in bare sediment was consistent with that of benthic denitrification. Transect water samplings of the wetlands showed different rainfall patterns (discrete versus continuous) affect the removal capability of the wetlands. During the discrete event sampling, the observed 15ε of NO3− (an average of 3.0 to 4.3 ‰) within the wetland falls between the experimental 15ε of benthic denitrification and assimilation; coincided with the decrease in NO3− concentrations, suggested that both denitrification and assimilation were important removal pathways. Depletion of δ15N-NO3− throughout the whole wetland system also suggested the influence of water column nitrification during this time. In contrast, during continuous rain events, no fractionation effect was observed within the wetland and was consistent with limited NO3− removal. The difference in fractionation factors within the wetland during different sampling conditions suggested that nitrate removal was highly likely limited by changes in overall nutrient inputs, residence time and water temperature which impeded biological uptake or removal. These highlight that consideration on sampling condition is crucial when assessing the efficacy of a wetland in removing nitrogen.
Details
- Title
- Stable isotopes of nitrate (δ15N and δ18O) as functional indicators of nitrogen processing in constructed wetlands
- Creators
- Keryn Roberts - Monash UniversityMichael Grace - Monash UniversityPerran Cook - Monash UniversityDirk Erler - Southern Cross UniversityWei-Wen Wong - Monash University
- Publication Details
- Science of The Total Environment, Vol.899, 165246
- Publisher
- Elsevier
- Identifiers
- 991013128413802368
- Copyright
- © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
- Academic Unit
- Faculty of Science and Engineering; Centre for Coastal Biogeochemistry; Science
- Language
- English
- Resource Type
- Journal article