Journal article
Spermatogonia survival in young ram lambs following irradiation, Busulfan or thermal treatment
Small ruminant research, Vol.166, pp.22-27
09/2018
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Abstract
Many alternatives to surgical castration have been explored to induce short or long term infertility in male animals. Comparatively few have been carefully evaluated in very young production animals. A comparison of four treatments known to deplete testicular cells in rodents (heating, cooling, chemotherapy, radiation) was undertaken using ram lambs.
Neither testicular cooling (0 °C) nor heating (45 °C) affected testis weights, tubule diameters or germ cell numbers. Low dose chemotherapy (Busulfan 4 mg/kg) treatment caused dramatic falls in white blood cells and platelets numbers which recovered within 3 weeks. Spermatogonia numbers were not significantly reduced (27% change).
The impact of irradiation doses (0–15 Gy) delivered with high precision to the testis by a 6 MV photon beam was assessed by serial biopsies. Sertoli cell numbers were depleted by 90% at 3 weeks in 15 Gy treated testes. Spermatogonia were depleted 8 weeks after irradiation with 9 Gy, 12 Gy and 15 Gy. By 13 weeks, only in the 15 Gy treated testes were spermatogonia and Sertoli cell numbers lower. At 13 weeks testis atrophy resulted in 3/6 lambs irradiated with 12 Gy or 15 Gy. Irradiation of very young lambs clearly compromised testis function, thermal treatment was ineffective and busulfan treatment resulted in minimal effects.
Details
- Title
- Spermatogonia survival in young ram lambs following irradiation, Busulfan or thermal treatment
- Creators
- Jeanette Olejnik - University of New EnglandNatalka Suchowerska - University of SydneyMuren Herrid - Commonwealth Scientific and Industrial Research OrganisationMichael Jackson - University of SydneyGeoff Hinch - University of New EnglandJonathan Hill - Commonwealth Scientific and Industrial Research Organisation
- Publication Details
- Small ruminant research, Vol.166, pp.22-27
- Publisher
- Elsevier B.V
- Identifiers
- 991013131521002368
- Copyright
- © 2018 Elsevier B.V. All rights reserved.
- Academic Unit
- Office of the Vice Chancellor
- Language
- English
- Resource Type
- Journal article