Spectral characterization, degradation behavior, quenching, and semi-quantification of fluoroquinolone antibiotics in the antibiotic-humic mixture using fluorescence spectroscopy

Nahin Mostofa Niloy; Fahmida Parvin; Shafi M. Tareq

doi:10.1016/j.scitotenv.2024.173346

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Spectral characterization, degradation behavior, quenching, and semi-quantification of fluoroquinolone antibiotics in the antibiotic-humic mixture using fluorescence spectroscopy

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Spectral characterization, degradation behavior, quenching, and semi-quantification of fluoroquinolone antibiotics in the antibiotic-humic mixture using fluorescence spectroscopy

Nahin Mostofa Niloy, Fahmida Parvin and Shafi M. Tareq

The Science of the total environment, Vol.935, pp.1-11

20/07/2024

DOI: https://doi.org/10.1016/j.scitotenv.2024.173346

PMID: 38777063

Appears in Recent Faculty of Science and Engineering Publications

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Abstract

fluoroquinolone antibiotics

fluorescence spectroscopy

excitation-emission matrix

fluorescence quenching

Antibiotics, one of the significant emerging contaminants, are intensifying their continual spread out into the environment and affecting human health and the ecosystem in the developing country Bangladesh. This study characterizes widely used fluoroquinolone (FQ) antibiotics, formulates the method to spectrally distinguish them from ubiquitous, and important reactive, adsorbent, and altering catalytic macromolecule humic substances (HS), and further quantifies them using fluorescence spectroscopy. The presence of identical fluorophore at Excitation/Emission = 225-230/285-295 nm wavelength, possession of fluorescence spectra at short emission wavelength (<350 nm) during 275 nm excitation, different emission maxima, and various fluorescing components in antibiotics identified through three-dimensional excitation-emission matrix (EEM) and parallel factor analysis (PARAFAC) models distinguished them from the humic substance as well as from each other. SternVolmer equation and its modified version were applied to identify quenching and binding capability, and fluorescence intensity quenching rate of antibiotics and humic in their mixture. Unlike poor and inconsistent quenching mechanisms of humic, FQ antibiotics reduced HS intensity throughout the entire photo-irradiation experiment affirming the functioning of the stable quenching methods. Static quenching of fluorophores was identified from the redshift of excited wavelength on the electronic ground state. Temperature differences during daylight and dark conditions played contrasting roles during the fluorescence quenching of FQ. Unique spectral response at emission wavelength < 350 nm during 275 nm excitation in FQ was considered as its least intensity in the antibiotic-humic mixture and was also used to formulate distinct spectral pattern of each FQ antibiotic. The study also identified the traces of FQ antibiotics with various intensities at different lakes in Bangladesh.

Details

Title: Spectral characterization, degradation behavior, quenching, and semi-quantification of fluoroquinolone antibiotics in the antibiotic-humic mixture using fluorescence spectroscopy
Creators: Nahin Mostofa Niloy - Jahangirnagar University
Fahmida Parvin - Jahangirnagar University
Shafi M. Tareq - Jahangirnagar University
Publication Details: The Science of the total environment, Vol.935, pp.1-11
Publisher: Elsevier
Number of pages: 11
Grant note: Shafi M Tareq is grateful to the Ministry of Education, Government of Bangladesh, for the research grant (LS 20201430).
Identifiers: 991013261010602368
Academic Unit: Faculty of Science and Engineering
Language: English
Resource Type: Journal article

Spectral characterization, degradation behavior, quenching, and semi-quantification of fluoroquinolone antibiotics in the antibiotic-humic mixture using fluorescence spectroscopy

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