Journal article
Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS
Bioanalysis, Vol.8(5), pp.397-411
2016
PMID: 26893268
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Source: InCites
Abstract
It has been suggested that each member of the family of vitamin D compounds may have different function(s). Therefore, selective quantification of each compound is important in clinical research.
Development and validation attempts of a simultaneous determination method of 12 vitamin D compounds in human blood using precolumn derivatization followed by LC-MS/MS is described. Internal standard calibration with 12 stable isotope labeled analogs was used to correct for matrix effects in MS detector.
Nine vitamin D compounds were quantifiable in blood samples with detection limits within femtomole levels. Serum (compared with plasma) was found to be a more suitable sample type, and protein precipitation (compared with saponification) a more effective extraction method for vitamin D assay.
Details
- Title
- Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS
- Creators
- Nur Sofiah Abu Kassim - University of QueenslandFabio P Gomes - University of QueenslandPaul Nicholas Shaw - University of QueenslandAmitha K Hewavitharana (Corresponding Author) - University of Queensland
- Publication Details
- Bioanalysis, Vol.8(5), pp.397-411
- Publisher
- England
- Identifiers
- 991012902099802368
- Academic Unit
- Faculty of Science and Engineering; Southern Cross Plant Science; Science
- Language
- English
- Resource Type
- Journal article