Journal article
Simultaneous Saccharification and Fermentation of Pretreated Eucalyptus grandis Under High Solids Loading
Industrial Biotechnology: the journal of biobased innovation to drive the global bioeconomy, Vol.13(3), pp.131-140
01/06/2017
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Abstract
Whole Eucalyptus grandis trees (including bark, branches and leaves) were investigated as a potential feedstock for bioethanol production. To demonstrate and maximize ethanol production, unwashed steam exploded wood chips (SEWC) were used as substrates in high solids load simultaneous saccharification fermentations (SSF) with an industrial Saccharomyces cerevisiae strain (Fali®). Under optimized SSF conditions—20 wt% solids, 60 filter paper units (FPU)/g glucan, and 36°C—ethanol titer and glucan-to-ethanol yields of 56 g/L and 90%, respectively, were achieved. Raising the SSF temperature to 39°C showed no observable benefit in final ethanol titers and/or yields. Addition of 3 wt% polyethylene glycol (PEG) further increased final ethanol titers and yields to 60 g/L and 95%, respectively, at a 30% lower cellulase dosage. Considering the mass balance of the best-performing SSF (20 wt% SEWC, 40 FPU Ctec 2/g glucan, 30 mg PEG/g dry solid at 36°C) process, the maximum ethanol attainable was estimated at 187 kg (85.8% yield) and 299 kg (95% yield) per dry metric ton of original and SEWC biomass, respectively.
Details
- Title
- Simultaneous Saccharification and Fermentation of Pretreated Eucalyptus grandis Under High Solids Loading
- Creators
- Shane McIntosh - NSW Department of Primary IndustriesJanice Palmer - NSW Department of Primary IndustriesZhanying Zhang - Queensland University of TechnologyWilliam Doherty - Queensland University of TechnologySyed S Yazdani - Jawaharlal Nehru UniversityRajeev K Sukumaran - National Institute for Interdisciplinary Science and TechnologyTony Vancov - NSW Department of Primary Industries
- Publication Details
- Industrial Biotechnology: the journal of biobased innovation to drive the global bioeconomy, Vol.13(3), pp.131-140
- Publisher
- Mary Ann Liebert, Inc. Publishers
- Identifiers
- 991013016097302368
- Copyright
- Copyright 2017, Mary Ann Liebert, Inc.
- Academic Unit
- Faculty of Science and Engineering; Science
- Language
- English
- Resource Type
- Journal article