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Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
Simultaneous Quantification of 17 Cannabinoids in Cannabis Inflorescence by Liquid Chromatography-Mass Spectrometry
Separations, Vol.9(4), pp.1-13
28/03/2022
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Source: InCites
Abstract
With an increasing appreciation for the unique pharmacological properties associated with distinct, individual cannabinoids of Cannabis sativa, there is demand for accurate and reliable quantification for a growing number of them. Although recent methods are based on highly selective chromatography-mass spectrometry technology, most are limited to a few cannabinoids, while relying on unnecessarily sophisticated and expensive ultra-high performance liquid chromatography and tandem mass spectrometry. Here we report an optimised, simple extraction method followed by a reliable and simple high performance liquid chromatography method for separation. The detection is performed using a time-of-flight mass spectrometer that is available in most natural products research laboratories. Due to the simplicity of instrumentation, and the robustness resulting from a high resolution in the chromatography of isobaric cannabinoids, the method is well suited for routine phytocannabinoid analysis for a range of applications. The method was validated in terms of detection and quantification limits, repeatability, and recoveries for a total of 17 cannabinoids: detection limits were in the range 11–520 pg when using a 1 µL sample injection volume, and the recovery percentages ranged from 85% to 108%. The validated method was subsequently applied to determine cannabinoid composition in the inflorescences of several medicinal Cannabis sativa varieties.
Details
- Title
- Simultaneous Quantification of 17 Cannabinoids in Cannabis Inflorescence by Liquid Chromatography-Mass Spectrometry
- Creators
- Amitha K Hewavitharana - Southern Cross UniversityFrancine Gloerfelt-Tarp - Southern Cross UniversityMatthew Nolan - Southern Cross UniversityBronwyn J Barkla - Southern Cross UniversitySarah Purdy - New South Wales Department of Primary IndustriesTobias Kretzschmar - Southern Cross University
- Publication Details
- Separations, Vol.9(4), pp.1-13
- Publisher
- MDPI AG
- Grant note
- This project received grant funding from the Australian Government via the Department of Industry, Science, Energy and Resources, as part of the Cooperative Research Centres Project (CRC-P) program—Round 7: Growing the medicinal cannabis industry—precision farming to pharmaceuticals.
- Identifiers
- 991013014198802368
- Copyright
- © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Academic Unit
- Faculty of Science and Engineering; Science; Southern Cross Plant Science
- Language
- English
- Resource Type
- Journal article