Journal article
Shortcomings of protein removal prior to high performance liquid chromatographic analysis-A case study using method development for BAY 11-7082
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Vol.834(1-2), pp.93-97
13/04/2006
PMID: 16530027
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Source: InCites
Abstract
During the analytical method development for BAY 11-7082 ((E)-3-[4-methylphenylsulfonyl]-2-propenenitrile), using HPLC-MS-MS and HPLC-UV, we observed that the protein removal process (both ultrafiltration and precipitation method using organic solvents) prior to HPLC brought about a significant reduction in the concentration of this compound. The use of a structurally similar internal standard, BAY 11-7085 ((E)-3-[4-t-butylphenylsulfonyl]-2-propenenitrile), was not effective in compensating for the loss of analyte as the extent of reduction was different to that of the analyte. We present here a systematic investigation of this problem and a new validated method for the determination of BAY 11-7082.
Details
- Title
- Shortcomings of protein removal prior to high performance liquid chromatographic analysis-A case study using method development for BAY 11-7082
- Creators
- A K Hewavitharana (Corresponding Author) - University of QueenslandC Hyde - Princess Alexandra HospitalR Thomas - Princess Alexandra HospitalP N Shaw - University of Queensland
- Publication Details
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Vol.834(1-2), pp.93-97
- Publisher
- Elsevier B.V.; Netherlands
- Identifiers
- 991012894198802368
- Academic Unit
- Faculty of Science and Engineering; Southern Cross Plant Science; Science
- Language
- English
- Resource Type
- Journal article