Short-term ecotoxicogenomic responses reveal early molecular mechanisms of pyraclostrobin toxicity in honey bees

Jaine da Luz Scheffer; Isabella Cristina de Castro Lippi; Yan Souza Lima; Juliana Sartori Lunardi; Marcus Vinícius Niz Alvarez; Ricardo de Oliveira Orsi

doi:10.1016/j.etap.2026.105004

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Short-term ecotoxicogenomic responses reveal early molecular mechanisms of pyraclostrobin toxicity in honey bees

Journal article

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Short-term ecotoxicogenomic responses reveal early molecular mechanisms of pyraclostrobin toxicity in honey bees

Jaine da Luz Scheffer, Isabella Cristina de Castro Lippi, Yan Souza Lima, Juliana Sartori Lunardi, Marcus Vinícius Niz Alvarez and Ricardo de Oliveira Orsi

Environmental toxicology and pharmacology, Vol.124, pp.1-9

06/2026

DOI: https://doi.org/10.1016/j.etap.2026.105004

PMID: 41887550

Appears in Recent Faculty of Science and Engineering Publications

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Abstract

Detoxification

Genes

Immunity

Pesticides

Pollinators

Transcriptome

Bees are essential to biodiversity and food production but are increasingly exposed to agricultural fungicides, such as pyraclostrobin (PYR), which is frequently detected in hive matrices. This study determined the oral lethal dose (LD₅₀) of PYR in Africanized Apis mellifera and evaluated early transcriptomic responses in forager bees following one and four-hour exposure to contaminated syrup. Head tissues were subjected to RNA sequencing. The LD₅₀ was 8.85 μg/bee, and a sublethal dose was selected for molecular analyses. After one hour, 432 genes were differentially expressed, with 104 shared between lethal and sublethal treatments. Upregulated genes were mainly associated with detoxification and immune pathways, indicating activation of xenobiotic processing and stress defence mechanisms. No significant transcriptional changes were observed after four hours. These findings demonstrate rapid molecular responses to PYR exposure and highlight the value of ecotoxicogenomics for detecting sublethal pesticide effects and informing risk assessment for pollinators.

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Title: Short-term ecotoxicogenomic responses reveal early molecular mechanisms of pyraclostrobin toxicity in honey bees
Creators: Jaine da Luz Scheffer - Universidade Estadual Paulista (Unesp)
Isabella Cristina de Castro Lippi - Southern Cross University
Yan Souza Lima - Universidade de São Paulo
Juliana Sartori Lunardi - Universidade Estadual Paulista (Unesp)
Marcus Vinícius Niz Alvarez - Universidade Estadual Paulista (Unesp)
Ricardo de Oliveira Orsi - Universidade Estadual Paulista (Unesp)
Publication Details: Environmental toxicology and pharmacology, Vol.124, pp.1-9
Publisher: Elsevier BV
Grant note: This research was supported by the State of São Paulo Research Foundation (FAPESP) [process 2020/10524-0] and the Federal Agency for Support and Evaluation of Graduate Education (CAPES) [finance code 001].
Identifiers: 991013370627002368
Academic Unit: Faculty of Science and Engineering; Science
Language: English
Resource Type: Journal article

Short-term ecotoxicogenomic responses reveal early molecular mechanisms of pyraclostrobin toxicity in honey bees

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