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Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
Semi-purified Antimicrobial Proteins from Oyster Hemolymph Inhibit Pneumococcal Infection
Marine biotechnology, Vol.26(5), pp.862-875
01/10/2024
PMID: 38430292
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Source: InCites
Abstract
Pneumococcal infections caused by Streptococcus pneumoniae are a leading cause of morbidity and mortality globally, particularly among children. The ability of S. pneumoniae to form enduring biofilms makes treatment inherently difficult, and options are further limited by emerging antibiotic resistance. The discovery of new antibiotics, particularly those with antibi-ofilm activity, is therefore increasingly important. Antimicrobial proteins and peptides (AMPs) from marine invertebrates are recognised as promising pharmacological leads. This study determined the in vitro antibacterial activity of hemolymph and unique protein fractions from an Australian oyster (Saccostrea glomerata) against multi-drug-resistant S. pneumoniae. We developed a successful method for hemolymph extraction and separation into 16 fractions by preparative HPLC. The strongest activity was observed in fraction 7: at 42 µg/mL protein, this fraction was bactericidal to S. pneumoniae and inhibited biofilm formation. Proteomic analysis showed that fraction 7 contained relatively high abundance of carbonic anhydrase, cofilin, cystatin B–like, and gelsolin-like proteins, while surrounding fractions, which showed lower or no antibacterial activity , contained these proteins in lower abundance or not at all. This work supports traditional medicinal uses of oysters and contributes to further research and development of novel hemolymph/AMP-based treatments for pneumococcal infections.
Details
- Title
- Semi-purified Antimicrobial Proteins from Oyster Hemolymph Inhibit Pneumococcal Infection
- Creators
- Kate Summer - Southern Cross UniversityBen Liu - Southern Cross UniversityQi Guo (Author) - Southern Cross UniversityBronwyn J Barkla (Author) - Southern Cross UniversityKirsten Benkendorff (Author) - Southern Cross University
- Publication Details
- Marine biotechnology, Vol.26(5), pp.862-875
- Publisher
- Springer New York LLC
- Grant note
- Financial support was provided by the Australian Research Training Program, SCU Faculty of Science and Engineering, SCU Faculty of Health, and the Malacological Research Society of Australasia.
- Identifiers
- 991013169913702368
- Copyright
- © Crown 2024. This article is licensed under a Creative Commons Attribution 4.0 International License. Open Access funding enabled and organized by CAUL and its Member Institutions.
- Academic Unit
- Southern Cross Analytical Research Services; Faculty of Science and Engineering; Science
- Language
- English
- Resource Type
- Journal article