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Journal article
Salt stress alters membrane lipid content and lipid biosynthesis pathways in the plasma membrane and tonoplast
Plant Physiology (Online), Vol.189(2), pp.805-826
06/2022
PMID: 35289902
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Abstract
Plant cell membranes are the sites of sensing and initiation of rapid responses to changing environmental factors including salinity stress. Understanding the mechanisms involved in membrane remodeling is important for studying salt tolerance in plants. This task remains challenging in complex tissue due to suboptimal subcellular membrane isolation techniques. Here, we capitalized on the use of a surface charge-based separation method, free flow electrophoresis, to isolate the tonoplast and plasma membrane from leaf tissue of the halophyte ice plant (Mesembryanthemum crystallinum L.). Results demonstrated a membrane-specific lipidomic remodeling in this plant under salt conditions, including an increased proportion of bilayer forming lipid phosphatidylcholine in the tonoplast and an increase in non-bilayer forming and negatively charged lipids (phosphatidylethanolamine and phosphatidylserine) in the plasma membrane. Quantitative proteomics showed salt-induced changes in proteins involved in fatty acid synthesis and desaturation, glycerolipid and sterol synthesis, as well as proteins involved in lipid signaling, binding, and trafficking. These results reveal an essential plant mechanism for membrane homeostasis wherein lipidome remodeling in response to salt stress contributes to maintaining the physiological function of individual subcellular compartments.
Details
- Title
- Salt stress alters membrane lipid content and lipid biosynthesis pathways in the plasma membrane and tonoplast
- Creators
- Qi Guo - Southern Cross UniversityLei Liu - Southern Cross UniversityThusitha W T Rupasinghe - University of MelbourneUte Roessner - University of MelbourneBronwyn J Barkla - Southern Cross University
- Publication Details
- Plant Physiology (Online), Vol.189(2), pp.805-826
- Publisher
- American Society of Plant Biologists
- Grant note
- This research was funded by Southern Cross University Higher Degree Research scholarship for Q.G
- Identifiers
- 991013004098202368
- Copyright
- © The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
- Academic Unit
- Science; Southern Cross Plant Science; Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article