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Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae
Journal of Water and Health, Vol.20(1), pp.83-91
01/2022
PMID: 35100156
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Source: InCites
Abstract
The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying the interaction of the enveloped bacteriophage Phi6, as a potential surrogated along with one of the most common amoebae in engineered aquatic environments, Vermamoeba vermiformis. Using microscopy, imaging flow cytometry and bacteriophage cell culture, our results imply that the SARS-CoV-2 surrogate triggers amoebic mitochondria and induced apoptosis to promote viral persistence in trophozoites. Furthermore, virus-infected amoebae were still infectious after 2 months within FLA cysts. These results suggest that amoebae could contribute to the environmental persistence of SARS-CoV-2, including disinfection processes. In addition, amoebae could be a successful model system for understanding respiratory virus-eukaryotic biology at the cellular and molecular levels.
Details
- Title
- SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae
- Creators
- Rafik Dey (Author) - University of AlbertaElena Dlusskaya (Author) - University of AlbertaNicholas Ashbolt (Author) - Southern Cross University
- Publication Details
- Journal of Water and Health, Vol.20(1), pp.83-91
- Publisher
- I W A Publishing
- Identifiers
- 991012964400402368
- Copyright
- This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits copying, adaptation and redistribution, provided the original work is properly cited (http://creativecommons.org/licenses/by/4.0/).
- Academic Unit
- Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article