Role of amoebae for survival and recovery of ‘non-culturable’ Helicobacter pylori cells in aquatic environments

Rafik Dey; Aja Rieger; Graham Banting; Nicholas J Ashbolt

doi:10.1093/femsec/fiaa182

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Role of amoebae for survival and recovery of ‘non-culturable’ Helicobacter pylori cells in aquatic environments

Journal article

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Role of amoebae for survival and recovery of ‘non-culturable’ Helicobacter pylori cells in aquatic environments

Rafik Dey, Aja Rieger, Graham Banting and Nicholas J Ashbolt

FEMS Microbiology Ecology, Vol.96(10), fiaa182

2020

DOI: https://doi.org/10.1093/femsec/fiaa182

PMID: 32897313

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Abstract

Helicobacter pylori

free-living amphizoic amoebae

intracellular multiplication

extracellular vesicles

phagosomal pH

bacterial recovery

ABSTRACT Helicobacter pylori is a fastidious Gram-negative bacterium that infects over half of the world's population, causing chronic gastritis and is a risk factor for stomach cancer. In developing and rural regions where prevalence rate exceeds 60%, persistence and waterborne transmission are often linked to poor sanitation conditions. Here we demonstrate that H. pylori not only survives but also replicates within acidified free-living amoebal phagosomes. Bacterial counts of the clinical isolate H. pylori G27 increased over 50-fold after three days in co-culture with amoebae. In contrast, a H. pylori mutant deficient in a cagPAI gene (cagE) showed little growth within amoebae, demonstrating the likely importance of a type IV secretion system in H. pylori for amoebal infection. We also demonstrate that H. pylori can be packaged by amoebae and released in extracellular vesicles. Furthermore, and for the first time, we successfully demonstrate the ability of two free-living amoebae to revert and recover viable but non-cultivable coccoid (VBNC)-H. pylori to a culturable state. Our studies provide evidence to support the hypothesis that amoebae and perhaps other free-living protozoa contribute to the replication and persistence of human-pathogenic H. pylori by providing a protected intracellular microenvironment for this pathogen to persist in natural aquatic environments and engineered water systems, thereby H. pylori potentially uses amoeba as a carrier and a vector of transmission.

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Title: Role of amoebae for survival and recovery of ‘non-culturable’ Helicobacter pylori cells in aquatic environments
Creators: Rafik Dey (Author) - School of Public Health, University of Alberta,11405-87 Avenue, Edmonton, Alberta T6G 1C9, Canada, Deparment of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada
Aja Rieger (Author) - Deparment of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada
Graham Banting (Author) - School of Public Health, University of Alberta,11405-87 Avenue, Edmonton, Alberta T6G 1C9, Canada
Nicholas J Ashbolt (Author) - School of Public Health, University of Alberta,11405-87 Avenue, Edmonton, Alberta T6G 1C9, Canada, Deparment of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada, Provincial Laboratory for Public Health (ProvLab), Alberta Health Services, Edmonton, Canada, School of Environmental, Sciense and Engineering, Southern Cross University, Lismore NSW, Australia
Publication Details: FEMS Microbiology Ecology, Vol.96(10), fiaa182
Identifiers: 991012925010602368
Academic Unit: Faculty of Science and Engineering; Science
Language: English
Resource Type: Journal article

Role of amoebae for survival and recovery of ‘non-culturable’ Helicobacter pylori cells in aquatic environments

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