Journal article
Ready to harvest? Spine colour predicts gonad index and gonad colour rating of a commercially important sea urchin
International Journal of Pediatric Otorhinolaryngology, Vol.120, pp.51-57
2019
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Abstract
<p><h3 id="x-x-sectitle0015">Objectives</h3> <p id="x-x-abspara0010">Regulatory T <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/lymphocyte" title="Learn more about Lymphocyte from ScienceDirect's AI-generated Topic Pages">lymphocytes</a> (Treg) have been linked to survival of commensal bacteria at mucosal sites, but their presence and role in <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/chronic-otitis-media" title="Learn more about Chronic Otitis Media from ScienceDirect's AI-generated Topic Pages">chronic otitis media</a> (COM) and their response to otopathogens has not been evaluated previously. We investigated the association between Treg lymphocytes and otopathogens in COM prone and non-COM prone children. <h3 id="x-x-sectitle0020">Methods</h3> <p id="x-x-abspara0015">Forty children, 2–7 years of age, scheduled for <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/adenoidectomy" title="Learn more about Adenoidectomy from ScienceDirect's AI-generated Topic Pages">adenoidectomy</a> were enrolled into COM (<em>n</em> = 20) or non-COM (<em>n</em> = 20) groups. <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/adenoid" title="Learn more about Adenoid from ScienceDirect's AI-generated Topic Pages">Adenoid</a> <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/biopsy" title="Learn more about Biopsy from ScienceDirect's AI-generated Topic Pages">biopsy</a> and <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/nasopharyngeal-aspiration" title="Learn more about Nasopharyngeal Aspiration from ScienceDirect's AI-generated Topic Pages">nasopharyngeal aspirate</a> bacteriology were assessed by conventional culture techniques. Peripheral blood and adenoid lymphocytes were stained with viability stain, <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/monoclonals" title="Learn more about Monoclonals from ScienceDirect's AI-generated Topic Pages">monoclonal</a> anti-CD19, anti-CD3, anti-CD4, anti-CD8, anti-CD25 and anti-CD127. Cells were stained intracellularly with monoclonal anti-FoxP3 and then quantified by <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/flow-cytometry" title="Learn more about Flow Cytometry from ScienceDirect's AI-generated Topic Pages">flow cytometry</a>. <h3 id="x-x-sectitle0025">Results</h3> <p id="x-x-abspara0020">Children with nasopharyngeal otopathogen-positive culture had significantly more circulating CD3+CD4+FoxP3+CD25hi+CD127lo+ lymphocytes (<em>M</em> = 4.4%) compared to culture-negative children (<em>M</em> = 3.1%, <em>p</em> = 0.005). Circulating CD19+ lymphocytes were significantly increased in children with positive <em><a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/moraxella-catarrhalis" title="Learn more about Moraxella catarrhalis from ScienceDirect's AI-generated Topic Pages">Moraxella catarrhalis</a></em> nasopharyngeal culture (<em>M</em> = 12.4%) compared to culture-negative children (<em>M</em> = 8.6%, <em>p</em> = 0.006). Adenoid-derived lymphocytes were not significantly different in children with any positive nasopharyngeal culture compared to negative culture. <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/lymphocyte-subpopulation" title="Learn more about Lymphocyte Subpopulation from ScienceDirect's AI-generated Topic Pages">Lymphocyte subsets</a>were not significantly different between COM and non-COM prone children. <h3 id="x-x-sectitle0030">Conclusion</h3> <p id="x-x-abspara0025">Clinically-detectable otopathogen nasopharyngeal culture is positively associated with Treg lymphocytes, potentially inducing suppressive effector responses to promote colonization and infection <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/chronicity" title="Learn more about Chronicity from ScienceDirect's AI-generated Topic Pages">chronicity</a>. This finding supports further investigation of Treg lymphocyte activity and influence on upper airway colonization of young children.</p>
Details
- Title
- Ready to harvest? Spine colour predicts gonad index and gonad colour rating of a commercially important sea urchin
- Creators
- Jessica Browne - Central Queensland UniversityEvan Howard Matthews - Mater Misericordiae Rockhampton Hospital, AustraliaAndrew William Taylor-RobinsonJennelle Maree Kyd - Swinburne University of Technology
- Publication Details
- International Journal of Pediatric Otorhinolaryngology, Vol.120, pp.51-57
- Identifiers
- 3978; 991012822061802368
- Academic Unit
- Faculty of Health; School of Health and Human Sciences; Human Sciences
- Resource Type
- Journal article