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AcceptedCC BY-NC-ND V4.0, Open Access
AcceptedCC BY-NC-ND V4.0, Open
Journal article
Pyrroloquinoline quinone influences intracellular alpha-synuclein aggregates
Experimental Results, Vol.4, pp.1-13
2023
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Abstract
Parkinson's disease (PD) is an irreversible neurodegenerative disorder clinically manifesting in uncontrolled motor symptoms. There are two primary hallmark features of Parkinson's disease - an irreversible loss of dopaminergic neurons of the substantia nigra pars compacta and formation of intracellular insoluble aggregates called Lewy bodies mostly composed of alpha-synuclein. Using a clinical improvements-first approach, we identified several clinical trials involving consumption of a specific diet or nutritional supplementation improved motor and non-motor functions. Here, we aimed to investigate if and how pyrroloquinoline quinone (PQQ) compound disrupts pre-formed alpha28 synuclein deposits using SH-SY5Y cells, widely used Parkinson’s Disease cellular model. SH-SY5Y neuroblastoma cells, incubated in presence of potassium chloride (KCl) to induce alpha30 synuclein protein aggregation, were treated with PQQ for up to 48 hours. Resulting aggregates were examined and quantified using confocal microscopy. Overall, nutritional compound PQQ reduced the average number and overall size of intracellular cytoplasmic alpha-synuclein aggregates in a PD cellular model.
Details
- Title
- Pyrroloquinoline quinone influences intracellular alpha-synuclein aggregates
- Creators
- Elizabeth Mountford - Endeavor College of Natural Health (Perth, Western Australia)Cynthia Mathew - University of CanberraReena Ghildyal - University of CanberraAndrea Bugarcic - Southern Cross University
- Publication Details
- Experimental Results, Vol.4, pp.1-13
- Publisher
- Cambridge University Press
- Identifiers
- 991013116173502368
- Copyright
- © The Author(s), 2023. Published by Cambridge University Press. This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence , (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- Academic Unit
- National Centre for Naturopathic Medicine
- Language
- English
- Resource Type
- Journal article