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Journal article
Predictors Linking Obesity and the Gut Microbiome (the PROMISE Study): Protocol and Recruitment Strategy for a Cross-Sectional Study on Pathways That Affect the Gut Microbiome and Its Impact on Obesity
JMIR Research Protocols, Vol.8(8), e14529
26/08/2019
PMCID: PMC6732966
PMID: 31452525
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Abstract
The prevalence of obesity has increased substantially over recent decades and is associated with considerable health inequalities. Although the causes of obesity are complex, key drivers include overconsumption of highly palatable, energy-dense, and nutrient-poor foods, which have a profound impact on the composition and function of the gut microbiome. Alterations to the microbiome may play a critical role in obesity by affecting energy extraction from food and subsequent energy metabolism and fat storage.
We report the study protocol and recruitment strategy of the PRedictors linking Obesity and the gut MIcrobiomE (PROMISE) study, which characterizes the gut microbiome in 2 populations with different metabolic disease risk (Pacific and European women) and different body fat profiles (normal and obese). It investigates (1) the role of gut microbiome composition and functionality in obesity and (2) the interactions between dietary intake; eating behavior; sweet, fat, and bitter taste perception; and sleep and physical activity; and their impact on the gut microbiome, metabolic and endocrine regulation, and body fat profiles.
Healthy Pacific and New Zealand (NZ) European women aged between 18 and 45 years from the Auckland region were recruited for this cross-sectional study. Participants were recruited such that half in each group had either a normal weight (body mass index [BMI] 18.5-24.9 kg/m
) or were obese (BMI ≥30.0 kg/m
). In addition to anthropometric measurements and assessment of the body fat content using dual-energy x-ray absorptiometry, participants completed sweet, fat, and bitter taste perception tests; food records; and sleep diaries; and they wore accelerometers to assess physical activity and sleep. Fasting blood samples were analyzed for metabolic and endocrine biomarkers and DNA extracted from fecal samples was analyzed by shotgun sequencing. Participants completed questionnaires on dietary intake, eating behavior, sleep, and physical activity. Data were analyzed using descriptive and multivariate regression methods to assess the associations between dietary intake, taste perception, sleep, physical activity, gut microbiome complexity and functionality, and host metabolic and body fat profiles.
Of the initial 351 women enrolled, 142 Pacific women and 162 NZ European women completed the study protocol. A partnership with a Pacific primary health and social services provider facilitated the recruitment of Pacific women, involving direct contact methods and networking within the Pacific communities. NZ European women were primarily recruited through Web-based methods and special interest Facebook pages.
This cross-sectional study will provide a wealth of data enabling the identification of distinct roles for diet, taste perception, sleep, and physical activity in women with different body fat profiles in modifying the gut microbiome and its impact on obesity and metabolic health. It will advance our understanding of the etiology of obesity and guide future intervention studies involving specific dietary approaches and microbiota-based therapies.
Australian New Zealand Clinical Trials Registry ACTRN12618000432213; https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=370874.
RR1-10.2196/14529.
Details
- Title
- Predictors Linking Obesity and the Gut Microbiome (the PROMISE Study): Protocol and Recruitment Strategy for a Cross-Sectional Study on Pathways That Affect the Gut Microbiome and Its Impact on Obesity
- Creators
- Sophie Kindleysides - School of Exercise, Sport and Nutrition, College of Health, Massey University, Auckland, New ZealandRozanne Kruger - School of Exercise, Sport and Nutrition, College of Health, Massey University, Auckland, New ZealandJeroen Douwes - Centre for Public Health Research, Massey University, Wellington, New ZealandGerald W Tannock - Riddet Centre of Research Excellence, Palmerston North, New ZealandNikki Renall - Riddet Centre of Research Excellence, Palmerston North, New ZealandJoanne Slater - School of Exercise, Sport and Nutrition, College of Health, Massey University, Auckland, New ZealandBlair Lawley - Microbiome Otago, University of Otago, Dunedin, New ZealandAnne-Thea McGill - School of Health & Human Sciences, Southern Cross University, East Lismore, AustraliaNiamh Brennan - School of Exercise, Sport and Nutrition, College of Health, Massey University, Auckland, New ZealandMoana Manukia - The Fono Health and Social Services, Auckland, New ZealandMarilize Richter - School of Exercise, Sport and Nutrition, College of Health, Massey University, Auckland, New ZealandRidvan Tupai-Firestone - Centre for Public Health Research, Massey University, Wellington, New ZealandT Leigh Signal - Sleep/Wake Research Centre, College of Health, Massey University, Wellington, New ZealandPhilippa Gander - Sleep/Wake Research Centre, College of Health, Massey University, Wellington, New ZealandStephen R Stannard - School of Exercise, Sport and Nutrition, College of Health, Massey University, Auckland, New ZealandBernhard H Breier - Riddet Centre of Research Excellence, Palmerston North, New Zealand
- Publication Details
- JMIR Research Protocols, Vol.8(8), e14529
- Publisher
- J M I R Publications, Inc.; Canada
- Identifiers
- 991012925070802368
- Copyright
- © Sophie Kindleysides, Rozanne Kruger, Jeroen Douwes, Gerald W Tannock, Nikki Renall, Joanne Slater, Blair Lawley, Anne-Thea McGill, Niamh Brennan, Moana Manukia, Marilize Richter, Ridvan Tupai-Firestone, T Leigh Signal, Philippa Gander, Stephen R Stannard, Bernhard H Breier. Originally published in JMIR Research Protocols (http://www.researchprotocols.org), 26.08.2019. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Research Protocols, is properly cited. The complete bibliographic information, a link to the original publication on http://www.researchprotocols.org, as well as this copyright and license information must be included.
- Academic Unit
- National Centre for Naturopathic Medicine; School of Health and Human Sciences; Faculty of Health
- Language
- English
- Resource Type
- Journal article