Journal article
Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution
Acta Tropica, Vol.226, pp.1-9
02/2022
PMID: 34890540
Appears in Recent Faculty of Health Publications
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Abstract
Acanthamoeba keratitis infection extends due to the growing number of contact lens users. Indigenous plants including Garcinia mangostana play a vital role in human health and well being. Many species of this plant have been reported with myriads of potent medicinal properties. However, the aims of this study were, for the first time, to isolate compounds from the flower of G. mangostana and to test their anti-Acanthamoeba and anti-adhesion activity against Acanthamoeba triangularis. Powdered flowers of G. mangostana were extracted and chromatographed on a silica gel column. The structures of the compounds were established with the aid of 1H NMR. More so, the anti-Acanthamoeba and anti-adhesion properties were tested on a 96-well polystyrene microtiter plate and soft contact lenses. Scanning electron microscope (SEM) was used to determine the features of A. triangularis on contact lenses. Eight pure compounds were obtained, namely 9-hydroxycalabaxanthone, tovophillin A, garcinone E, garcinone B, α-mangostin, gartinin, 8-deoxygartinin and γ-mangostin. The extract and pure compounds exhibited anti-Acanthamoeba activity with MIC values in the range of 0.25–1 mg/mL. In addition, the extract and α-mangostin displayed significant activity against the adhesion of A. triangularis trophozoites both in polystyrene plate and in contact lenses at 0.5 × MIC (0.25 mg/mL). Furthermore, α-mangostin has the potential to remove A. triangularis adhesion in contact lenses similar to a commercial multipurpose solution (MPS). SEM study confirmed that crude extract and α-mangostin are effective as solutions for contact lenses, which removed A. triangularis trophozoites within 24 h. Alpha-mangostin was non-toxic to Vero cells at a concentration below 39 μM in 24 h. Crude extract of G. mangostana flower and its α-mangostin serve as candidate compounds in the treatment of Acanthamoeba infection or as lens care solution, since they can be used as a source of natural products against Acanthamoeba and virulence factor associated with the adhesion of A. triangularis.
Details
- Title
- Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution
- Creators
- Suthinee Sangkanu - Walailak UniversityWatcharapong Mitsuwan - Walailak UniversityTooba Mahboob - Walailak UniversityWilawan Mahabusarakam - Prince of Songkla UniversitySopa Chewchanwuttiwong - Prince of Songkla UniversityPanisara Siphakdi - Prince of Songkla UniversityTajudeen O Jimoh - Chulalongkorn UniversityPolrat Wilairatana - Mahidol UniversityKarma G Dolma - Department of Microbiology, Sikkim Manipal Institute of Medical Sciences, Sikkim, IndiaMaria de Lourdes Pereira - University of Aveiro (UA)Mohammed Rahmatullah - University of Development AlternativeChristophe Wiart - Universiti Malaysia SabahRoghayeh Norouzi - University of TabrizAbolghasem Siyadatpanah - Birjand University of Medical SciencesPolydor Ngoy Mutombo - Southern Cross UniversityVeeranoot Nissapatorn - Walailak University
- Publication Details
- Acta Tropica, Vol.226, pp.1-9
- Publisher
- Elsevier BV
- Grant note
- The Royal Patronage of Her Royal Highness Princess Maha Chakri Sirindhorn - Botanical Garden of Walailak University, Nakhon Si Thammarat, under the project entitled: Medicinal under-exploited Thai native plant against Acanthamoeba, Leishmania donovani, and Plasmodium falciparum - Toward South East Asia collaboration initiative (WUBG 031-2565).
- Identifiers
- 991012988296102368
- Copyright
- © 2021 Elsevier B.V. All rights reserved.
- Academic Unit
- Faculty of Health; National Centre for Naturopathic Medicine
- Language
- English
- Resource Type
- Journal article