Journal article
Phylogenetic relationships, virulence and antimicrobial resistance properties of Klebsiella sp. isolated from pet turtles in Korea
Letters in Applied Microbiology, Vol.70(2), pp.71-78
2019
PMID: 31671218
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Abstract
<p><em>Klebsiella</em> sp. are responsible for a multitude of infectious diseases in both humans and animals. In this study, phylogenetic relationships, virulence and antimicrobial resistance gene properties of 16 <em>Klebsiella</em> sp. isolated from 49 pet turtles were investigated. The isolates including <em>Klebsiella oxytoca</em> (n = 13) and <em>Klebsiella pneumoniae</em> (n = 3) were identified using 16S rRNA gene sequencing and each species formed distinct clusters in the neighbour-joining phylogenetic tree. The prevalence of virulence genes including <em>ureC</em> (100%) and <em>kfu</em> (68·75%) was observed among the isolates using Polymerase chain reaction (PCR) assay. The <em>fimH, mrkD</em> and <em>rmpA</em> genes were detected in all <em>K. pneumoniae</em> while these were absent in every <em>K. oxytoca</em> isolate. In antimicrobial susceptibility testing, high resistance rates were observed against ampicillin (100%) and cephalothin (62·50%). The resistance rates against imipenem, tetracycline, trimethoprim/sulfamethoxazole, nalidixic acid and ciprofloxacin were 12·50, 12·50, 12·50, 6·25 and 6·25% respectively. The presence of antimicrobial resistance genes such as plasmid-mediated quinolone resistance (PMQR) [<em>qnrB</em> (37·50%), <em>qnrA</em> (31·25%), <em>qnrS</em> (12·50%) and <em>aac(6')-Ib-cr</em> (12·50%)], extended-spectrum β-lactamase (ESBL) [<em>blaCTX-M</em> (18·75%)], β-lactamase [<em>blaSHV-1</em> (18·75%)] and tetracycline resistance [<em>tetE</em> (12·50%)] was observed. The results revealed that pet turtle-borne <em>Klebsiella</em> sp. may carry different types of virulence and antimicrobial resistance genes which represents a potential threat to public health.</p>
<p>SIGNIFICANCE AND IMPACT OF THE STUDY:</p>
<p><em>Klebsiella</em> sp. are nonmotile Gram-negative bacteria that are found in different environments. The virulence and antimicrobial resistance properties of pet turtle-borne <em>Klebsiella</em> sp. have not been studied before. Phylogenetic relationships, virulence traits and antimicrobial resistance profiles of pet turtle-borne <em>Klebsiella</em> sp. were characterized for the first time in Korea. Multiple virulence and antimicrobial resistance genes were observed among the isolates. The occurrence of virulence and antimicrobial resistance determinants in <em>Klebsiella</em> sp. may represent a potential threat to public health.
Details
- Title
- Phylogenetic relationships, virulence and antimicrobial resistance properties of Klebsiella sp. isolated from pet turtles in Korea
- Creators
- S Hossain - Chungbuk National UniversityB C J De Silva - Southern Cross University, Southern Cross Analytical Research ServicesP S Dahanayake - Chungbuk National UniversityGang‐Joon Heo (Corresponding Author) - Chungbuk National University
- Publication Details
- Letters in Applied Microbiology, Vol.70(2), pp.71-78
- Publisher
- Society for Applied Microbiology
- Identifiers
- 991012900099902368
- Academic Unit
- Southern Cross Analytical Research Services; Faculty of Science and Engineering; School of Environment, Science and Engineering
- Language
- English
- Resource Type
- Journal article