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Published (Version of record)CC BY-NC V4.0, Open Access
Published (Version of record)CC BY-NC V4.0, Open
Journal article
Overexpression of a wheat α-amylase type 2 impact on starch metabolism and abscisic acid sensitivity during grain germination
The Plant Journal, Vol.108(2), pp.378-393
10/2021
PMID: 34312931
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Source: InCites
Abstract
Summary
Despite being of vital importance for seed establishment and grain quality, starch degradation remains poorly understood in organs such as cereal or legume seeds. In cereals, starch degradation requires the synergetic action of different isoforms of α-amylases. Ubiquitous overexpression of TaAmy2 resulted in a 2.0–437.6-fold increase of total α-amylase activity in developing leaf and harvested grains. These increases led to dramatic alterations of starch visco-properties and augmentation of soluble carbohydrate levels (mainly sucrose and α-gluco-oligosaccharide) in grain. Interestingly, the overexpression of TaAMY2 led to an absence of dormancy in ripened grain due to abscisic acid (ABA) insensitivity. Using an allosteric α-amylase inhibitor (acarbose), we demonstrated that ABA insensitivity was due to the increased soluble carbohydrate generated by the α-amylase excess. Independent from the TaAMY2 overexpression, inhibition of α-amylase during germination led to the accumulation of soluble α-gluco-oligosaccharides without affecting the first stage of germination. These findings support the hypotheses that (i) endosperm sugar may overcome ABA signalling and promote sprouting, and (ii) α-amylase may not be required for the initial stage of grain germination, an observation that questions the function of the amylolytic enzyme in the starch degradation process during germination.
Details
- Title
- Overexpression of a wheat α-amylase type 2 impact on starch metabolism and abscisic acid sensitivity during grain germination
- Creators
- Qin Zhang - Agriculture and FoodJenifer Pritchard - Agriculture and FoodJos Mieog - Agriculture and FoodKeren Byrne - Agriculture and FoodMichelle L Colgrave - Agriculture and FoodJi-Rui Wang - Sichuan Agricultural UniversityJean-Philippe F Ral - Agriculture and Food
- Publication Details
- The Plant Journal, Vol.108(2), pp.378-393
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Number of pages
- 16
- Grant note
- Financial support for the research was provided by the CSIRO Research Office and the Chinese Scholarship Council mobility program.
- Identifiers
- 991012961200402368
- Copyright
- © 2021 Commonwealth of Australia. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution NonCommercial License,which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
- Academic Unit
- Faculty of Science and Engineering; School of Environment, Science and Engineering; Science; Southern Cross Plant Science
- Language
- English
- Resource Type
- Journal article