Optimisation of a microsatellite enrichment technique in saccharum spp.

The creation of enriched microsatellite libraries can ensure an abundant supply of microsatellite sequences at a considerably reduced cost. We report here the modification to an existing enrichment protocol and the optimisation of this technique for use on specific plant species, in this case, sugarcane. Using this optimised protocol, we achieved a 40% increase in the level of enrichment.