Journal article
Off-line measurement of carbon stable isotopes of trisaccharides, disaccharides, glucose and fructose in honey samples
Isotopes in environmental and health studies, Vol.62(2), pp.1-5
05/2026
PMID: 40987484
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Abstract
Online liquid chromatography (LC) followed by isotope ratio mass spectrometry (IRMS) is a trusted technique to detect honey adulteration based on the stable carbon isotope composition ( δC-13) of trisaccharides, disaccharides, glucose and fructose. However, LC-IRMS demands specialised analytical setups not commonly found in most isotope labs. Here we investigate the use of off-line LC followed by elemental analysis (EA)-IRMS as an alternative employing more commonly available instruments. Precision and accuracy were excellent for fructose and glucose (error < 0.1 mUr), but less so for disaccharides and trisaccharides, which are similar results to online LC-IRMS. Therefore, offline LC-EA-IRMS can be a viable alternative for the analysis of honey purity employing stable carbon isotopes.
Details
- Title
- Off-line measurement of carbon stable isotopes of trisaccharides, disaccharides, glucose and fructose in honey samples
- Creators
- Matheus C. Carvalho - Southern Cross University
- Publication Details
- Isotopes in environmental and health studies, Vol.62(2), pp.1-5
- Publisher
- Taylor & Francis
- Number of pages
- 5
- Identifiers
- 991013372626102368
- Copyright
- © 2025 The Author(s).
- Academic Unit
- Research Infrastructure and Operations
- Language
- English
- Resource Type
- Journal article