Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells

Marta Trevisan; Veronica Di Antonio; Annalisa Radeghieri; Giorgio Palù; Reena Ghildyal; Gualtiero Alvisi

doi:10.3390/v10030109

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Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells

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Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells

Marta Trevisan, Veronica Di Antonio, Annalisa Radeghieri, Giorgio Palù, Reena Ghildyal and Gualtiero Alvisi

Viruses, Vol.10(3), pp.1-13

03/03/2018

DOI: https://doi.org/10.3390/v10030109

PMCID: PMC5869502

PMID: 29510513

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Abstract

virus assembly

Bioluminescence resonance energy transfer (BRET)

confocal microscopy

RSV M protein

Respiratory syncytial virus (RSV) is an important human pathogen, which infects respiratory tract epithelial cells causing bronchiolitis and pneumonia in children and the elderly. Recent studies have linked RSV matrix (M) ability to self-interaction and viral budding. However, RSV M has been crystalized both as a monomer and a dimer, and no formal proof exists to date that it forms dimers in cells. Here, by using a combination of confocal laser scanning microscopy and bioluminescent resonant energy transfer applied to differently tagged deletion mutants of RSV M, we show that the protein can self-interact in living mammalian cells and that both the N and C-terminus of the protein are strictly required for the process, consistent with the reported dimeric crystal structure

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Title: Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells
Creators: Marta Trevisan - University of Padua
Veronica Di Antonio - University of Padua
Annalisa Radeghieri - University of Brescia
Giorgio Palù - University of Padua
Reena Ghildyal - University of Canberra
Gualtiero Alvisi - University of Padua
Publication Details: Viruses, Vol.10(3), pp.1-13
Publisher: MDPI AG
Identifiers: 991013036326902368
Copyright: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Academic Unit: National Centre for Naturopathic Medicine
Language: English
Resource Type: Journal article

Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells

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