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Journal article
Molecular Identification of Two Novel Munc-18 Isoforms Expressed in Non-neuronal Tissues (∗)
The Journal of Biological Chemistry, Vol.270(11), pp.5857-5863
17/03/1995
PMID: 7890715
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Abstract
Munc-18, also known as n-Sec1 or rbSec1, is a syntaxin-binding protein thought to play a role in regulating synaptic vesicle exocytosis. Although a gene family of syntaxins has been identified, only a limited subset bind to Munc-18. This implicates the existence of other mammalian Munc-18 homologues that may be involved in a range of vesicle transport reactions. The purpose of the present study was to identify other members of the Munc-18 family by cDNA cloning. Three distinct Munc-18 isoforms, Munc-18a, previously identified in neuronal tissue, and two novel isoforms, Munc-18b and Munc-18c, were isolated from a 3T3-L1 adipocyte cDNA library by screening with a rat brain Munc-18 DNA probe. Munc-18a is identical to Munc-18 and by Northern analysis is expressed predominantly in brain and to a lesser extent in testis and 3T3-L1 cells. Munc-18b is 62% identical to Munc-18 at the amino acid level and is expressed in testis, intestine, kidney, rat adipose tissue, and 3T3-L1 cells. Munc-18c is 51% identical to Munc-18 and is ubiquitously expressed. It is likely, based on these findings, that unique Munc-18/syntaxin interactions may play an important role in generating a combinatorial mechanism for the regulation of vesicle transport in mammalian cells.
Details
- Title
- Molecular Identification of Two Novel Munc-18 Isoforms Expressed in Non-neuronal Tissues (∗)
- Creators
- Judy T Tellam - University of QueenslandShane McIntosh - University of QueenslandDavid E James - University of Queensland
- Publication Details
- The Journal of Biological Chemistry, Vol.270(11), pp.5857-5863
- Publisher
- American Society for Biochemistry and Molecular Biology, Inc.
- Identifiers
- 991013019534302368
- Copyright
- This is an Open Access article under the CC BY license
- Academic Unit
- Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article