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Published (Version of record)CC BY-NC-ND V4.0, Open Access
Published (Version of record)CC BY-NC-ND V4.0, Open
Journal article
Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa
Plant Physiology, Vol.186(3), pp.1616-1631
07/2021
PMCID: PMC8260142
PMID: 33831190
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Abstract
Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) mutant, braA.sgn3.a-1, which accumulates twice as much Mg and a third more Ca in its leaves. We mapped braA.sgn3.a to a single recessive locus using a forward ionomic screen of chemically mutagenized lines with subsequent backcrossing and linked-read sequencing of second back-crossed, second filial generation (BC2F2) segregants. Confocal imaging revealed a disrupted root endodermal diffusion barrier, consistent with SGN3 encoding a receptor-like kinase required for normal formation of Casparian strips, as reported in thale cress (Arabidopsis thaliana). Analysis of the spatial distribution of elements showed elevated extracellular Mg concentrations in leaves of braA.sgn3.a-1, hypothesized to result from preferential export of excessive Mg from cells to ensure suitable cellular concentrations. This work confirms a conserved role of SGN3 in controlling nutrient homeostasis in B. rapa, and reveals mechanisms by which plants are able to deal with perturbed shoot element concentrations resulting from a “leaky” root endodermal barrier. Characterization of variation in leaf Mg and Ca accumulation across a mutagenized population of B. rapa shows promise for using such populations in breeding programs to increase edible concentrations of essential human and animal nutrients.
Details
- Title
- Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa
- Creators
- Thomas D Alcock - University of NottinghamCatherine L Thomas - University of NottinghamSeosamh Ó Lochlainn - University of NottinghamPaula Pongrac - Jožef Stefan InstituteMichael Wilson - University of NottinghamChristopher Moore - University of NottinghamGuilhem Reyt - University of NottinghamKatarina Vogel-Mikuš - Jožef Stefan InstituteMitja Kelemen - Jožef Stefan InstituteRory Hayden - University of NottinghamLolita Wilson - University of NottinghamPauline Stephenson - Department of Crop Genetics, John Innes Centre, Norwich Research ParkLars Østergaard - Department of Crop Genetics, John Innes Centre, Norwich Research ParkJudith A Irwin - Department of Crop Genetics, John Innes Centre, Norwich Research ParkJohn P Hammond - University of ReadingGraham J King - Southern Cross UniversityDavid E Salt - University of NottinghamNeil S Graham - University of NottinghamPhilip J White - Ecological Sciences, The James Hutton Institute, InvergowrieMartin R Broadley - University of Nottingham
- Publication Details
- Plant Physiology, Vol.186(3), pp.1616-1631
- Publisher
- American Society of Plant Biologists
- Grant note
- This work was supported by a series of projects and studentships: 1. The UK Biotechnology and Biological Sciences Research Council (BBSRC) and the UK Natural Environment Research Council (NERC), through a Sustainable Agriculture Research and Innovation Club (SARIC) project, Magnesium Network (MAG-NET): Integrating Soil-Crop-Animal Pathways to Improve Ruminant Health [grant number BB/N004302/1]. 2. The Future Food Beacon of Excellence, University of Nottingham, UK (T.D.A., G.R.). 3. The BBSRC, through a studentship funded by the Renewable Industrial Products from Rapeseed (RIPR) Program (T.D.A.) [grant number BB/L002124/1]. 4. The BBSRC, through a studentship (CLT) funded by a Crop Improvement Research Club (CIRC) project, Delivering Low-Cost, High-Throughput Root Phenotyping Screens for Arable Crops [grant number BB/J019631/1]. 5. The BBSRC, through an Industry Partnering Award, Biofortifying Brassica with Calcium (Ca) and Magnesium (Mg) for Human Health [grant number BB-G013969-1]. 6. The BBSRC, through a Targeted Priority Studentship in Crop Science (S.Ó.), Improving the Zinc (Zn) Composition of Brassica Crops [grant number BBSSE200613215]. 7. P.J.W. was supported by the Rural and Environment Science and Analytical Services Division (RESAS) of the Scottish Government. P.P., K.V.-M., and M.K. were supported by the Slovenian Research Agency through programs P1-0212 and P1-0112 and projects N7-0077, N1-0105, N1-0090, J7-9418, and J7-9398.
- Identifiers
- 991012961195902368
- Copyright
- © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
- Academic Unit
- Faculty of Science and Engineering; School of Environment, Science and Engineering; Science; Southern Cross Plant Science
- Language
- English
- Resource Type
- Journal article