LC-MS-based metabolomics study of marine bacterial secondary metabolite and antibiotic production in Salinispora arenicola

Utpal Bose; Amitha K Hewavitharana; Yi Kai Ng; Paul Nicholas Shaw; John A Fuerst; Mark P Hodson

doi:10.3390/md13010249

Back

LC-MS-based metabolomics study of marine bacterial secondary metabolite and antibiotic production in Salinispora arenicola

Journal article

Open access

Peer reviewed

LC-MS-based metabolomics study of marine bacterial secondary metabolite and antibiotic production in Salinispora arenicola

Utpal Bose, Amitha K Hewavitharana, Yi Kai Ng, Paul Nicholas Shaw, John A Fuerst and Mark P Hodson

Marine drugs, Vol.13(1), pp.249-266

2015

DOI: https://doi.org/10.3390/md13010249

PMCID: PMC4306935

PMID: 25574739

Files and links (2)

pdf

LC-MS-based metabolomics study of marine bacterial secondary metabolite and antibiotic production in Salinispora arenicola2.79 MBDownload View

Published (Version of record)CC BY V4.0, Open Access

url

https://doi.org/10.3390/md13010249View

Published (Version of record)CC BY V4.0, Open

Metrics

5 File views/ downloads

27 Record Views

41 Times Cited - Web of Science

See more details

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Abstract

Rifamycins - metabolism

Metabolomics

Seawater - microbiology

Animals

Mass Spectrometry

Rifamycins - isolation & purification

Chromatography, Liquid

Micromonosporaceae - metabolism

Porifera - microbiology

Anti-Bacterial Agents - isolation & purification

Micromonosporaceae - chemistry

An LC-MS-based metabolomics approach was used to characterise the variation in secondary metabolite production due to changes in the salt content of the growth media as well as across different growth periods (incubation times). We used metabolomics as a tool to investigate the production of rifamycins (antibiotics) and other secondary metabolites in the obligate marine actinobacterial species Salinispora arenicola, isolated from Great Barrier Reef (GBR) sponges, at two defined salt concentrations and over three different incubation periods. The results indicated that a 14 day incubation period is optimal for the maximum production of rifamycin B, whereas rifamycin S and W achieve their maximum concentration at 29 days. A "chemical profile" link between the days of incubation and the salt concentration of the growth medium was shown to exist and reliably represents a critical point for selection of growth medium and harvest time.

Details

Title: LC-MS-based metabolomics study of marine bacterial secondary metabolite and antibiotic production in Salinispora arenicola
Creators: Utpal Bose - University of Queensland
Amitha K Hewavitharana - University of Queensland
Yi Kai Ng - University of Queensland
Paul Nicholas Shaw - University of Queensland
John A Fuerst - University of Queensland
Mark P Hodson (Corresponding Author) - University of Queensland
Publication Details: Marine drugs, Vol.13(1), pp.249-266
Publisher: Switzerland
Identifiers: 991012893699202368
Academic Unit: Faculty of Science and Engineering; Southern Cross Plant Science; Science
Language: English
Resource Type: Journal article

LC-MS-based metabolomics study of marine bacterial secondary metabolite and antibiotic production in Salinispora arenicola

Files and links (2)

Metrics

UN Sustainable Development Goals (SDGs)

Abstract

Details

Southern Cross University Social media