Journal article
Isolation, characterisation and cross amplification of thirteen microsatellite loci for coral endo-symbiotic dinoflagellates (Symbiodinium clade C)
Conservation Genetics Resources, Vol.1(1), pp.199-203
12/2009
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Abstract
The health and productivity of coral reefs is underpinned by the symbiosis between corals and dinoflagellates (Symbiodinium spp.). To enable population genetic analyses of Symbiodinium spp, required for coral reef conservation, seven microsatellite loci were isolated from Symbiodinium clade C from the Great Barrier Reef, Australia. These microsatellite primer pairs consistently amplified between 1 and 8 alleles per coral host colony, with mean number of alleles ranging from 1.9 to 4.0 and generally high genetic diversities (Shannon’s Index = 0.71–2.76). The novel microsatellite loci amplified between 1 and 10 alleles in four other C strains, but did not amplify a D strain. Three of six previously published clade C microsatellite loci amplified 1–6 alleles in two or more of five C strains tested. The primers and cross amplifications presented here therefore provide a useful tool for elucidating the population genetic structure of clade C Symbiodinium populations.
Details
- Title
- Isolation, characterisation and cross amplification of thirteen microsatellite loci for coral endo-symbiotic dinoflagellates (Symbiodinium clade C)
- Creators
- Line Bay - Centre for Marine Microbiology and Genetics Australian Institute of Marine Science PMB #3 Townsville QLD 4810 AustraliaEmily Howells - AIMS@JCU James Cook University Townsville QLD 4810 AustraliaMadeleine van Oppen - Centre for Marine Microbiology and Genetics Australian Institute of Marine Science PMB #3 Townsville QLD 4810 Australia
- Publication Details
- Conservation Genetics Resources, Vol.1(1), pp.199-203
- Publisher
- Springer Netherlands
- Identifiers
- 991012966168002368
- Academic Unit
- National Marine Science Centre
- Language
- English
- Resource Type
- Journal article