Inhibitory effects of 12 hemostatic Chinese herbs on nitric oxide production in LPS-activated RAW264.7 macrophages

OBJECTIVE: To investigate the inhibitory effects of 12 hemostatic Chinese herbs on Nitric Oxide production in lipopolysaccharide(LPS)activated RAW 264.7 Macrophages. METHODS: Nitric Oxide production was determined with Griess assay after 22 hrs stimulation of 1 μ g· mL-1 LPS on RAW 264.7.Nitrite concentrations were determined by comparison with a sodium nitrite standard curve. RESULTS: The markedly increased production of nitrite by stimulation of RAW 264.7 macrophages with LPS was inhibited significantly(P0.05)by:Panax Notoginseng,Sanguisorba Officinalis,Agrimonia Pilosa,Sophora Japonica,Artemisia Argyi,Typha Angustifolia,Platycladus Orientalis and Imperata Cylindrical. CONCLUSION: The hemostatic action of 12 herbs may be related to their inhibitory effect on Nitric Oxide production