Journal article
Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug‐resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata)
Letters in Applied Microbiology, Vol.69(1), pp.2-10
2019
PMID: 30980564
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Abstract
<p><em>Aeromonas</em> sp. are opportunistic pathogenic bacteria which are associated with various diseases in ornamental fish, aquaculture raised species and wild fisheries. In our study, antimicrobial resistance patterns, antimicrobial resistance genes and class 1 integron gene cassettes of 52 guppy‐borne <em>Aeromonas</em> sp. were examined. The isolates were identified as <em>A. veronii</em> (n = 34), <em>A. dhakensis</em> (n = 10), <em>A. hydrophila</em> (n = 3), <em>A. caviae</em> (n = 3) and <em>A. enteropelogenes</em> (n = 2) by <em>gyrB</em> gene sequencing. Every isolate was resistant to at least four antimicrobials in disc diffusion test. The resistance to amoxicillin, nalidixic acid and oxytetracycline was 100% among the tested isolates. 92·30, 76·92, 71·15, 51·92, 51·92 and 50·00% of the isolates were resistant to ampicillin, rifampicin, imipenem, cephalothin, tetracycline and trimethoprim respectively. The multiple antibiotic resistance index values ranged from 0·28 to 0·67. PCR amplification of antimicrobial resistance genes implied the occurrence of tetracycline resistance (<em>tetA</em> (65·39%), <em>tetE</em> (25·00%) and <em>tetB</em> (15·38%)), plasmid‐mediated quinolone resistance (<em>qnrS</em> (26·92%) and <em>qnrB</em> (17·31%)) and aminoglycoside resistance (<em>aphaAI‐IAB</em> (7·69%) and <em>aac (6′)‐Ib</em> (3·84%)) genes in the isolates. The <em>IntI</em> gene was positive for 36·54% of the isolates and four class 1 integron gene cassette profiles (<em>aadA2, qacE2‐orfD, aadA2‐catB2</em> and <em>dfrA12‐aadA2</em>) were identified. These data suggest that ornamental guppy can be a reservoir of multidrug‐resistant <em>Aeromonas</em> sp. which comprise different antimicrobial resistance genes and class 1 integrons.</p>
<p>Significance and Impact of the Study</em></p>
<p>Antimicrobial resistance genes and integron gene cassettes of ornamental fish‐borne aeromonads are poorly studied. The antimicrobial resistance patterns, antimicrobial resistance genes and class 1 integron gene cassettes of <em>Aeromonas</em> sp. isolated from ornamental guppy were characterized for the first time in Korea. The incidence of different antimicrobial resistance genes and class 1 integron gene cassettes were observed in multidrug‐resistant <em>Aeromonas</em> isolates. This result suggests that better management practices are necessary to prevent and address the serious consequences of indiscriminate and inappropriate antimicrobial use, and the distribution of multidrug‐resistant bacteria.</p>
Details
- Title
- Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug‐resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata)
- Creators
- S Hossain - Chungbuk National UniversityB C J De Silva - Southern Cross University, Southern Cross Analytical Research ServicesM V K S Wickramanayake - Chungbuk National UniversityP S Dahanayake - Chungbuk National UniversityS.H.M.P Wimalasena - Chungbuk National UniversityGang‐Joon Heo (Corresponding Author) - Chungbuk National University
- Publication Details
- Letters in Applied Microbiology, Vol.69(1), pp.2-10
- Publisher
- Society for Applied Microbiology
- Identifiers
- 991012900099802368
- Academic Unit
- Southern Cross Analytical Research Services; Faculty of Science and Engineering; School of Environment, Science and Engineering
- Language
- English
- Resource Type
- Journal article