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Published (Version of record)CC BY-NC-ND V4.0, Open Access
Published (Version of record)CC BY-NC-ND V4.0, Open
Journal article
In vitro and in silico approach of fungal growth inhibition by Trichoderma asperellum HbGT6-07 derived volatile organic compounds
Arabian journal of chemistry, Vol.14(9), pp.1-21
01/09/2021
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Abstract
The species of Trichoderma are one of the most frequently used natural biocontrol agent. This study, we identified isolate HbGT6-07 of Trichoderma asperellum and evaluated the antimicrobial effects both in vitro and in silico approaches. Tested 10% concentrated culture filtrate of HbGT6-07 inhibited 98% of colony radial growth in B. cinerea (B05.10) as well as 91% of S. sclerotiorum (A367). HbGT6-07 was detected to produce volatile organic compounds (VOCs) with antifungal activity. In in-vitro dish-within-dish method (DwD), The HbGT6-07 VOCs effectively reduced colonial diameter, growth rate and sclerotia production by two virulent fungal pathogens. Moreover, the hyphal fragments of HbGT6-07 demonstrated successful mycelia growth suppression (97%) against infection oilseed rape leaves by hyphae of the two virulent fungal pathogens through competition. The mixed culture assay, exhibited that the isolate T. asperellum HbGT6-07 was significantly reduced the production and weight of sclerotia. The GC-MS analysis identified 32 VOCs derived from HbGT6-07. In addition, VOCs derived from HbGT6-07 were assessed against targeted protein of three fungal species; Aspergillus oryzae, Saccharomyces cerevisiae, Candida albicans via molecular docking. Butylated hydroxytolune and Beta-Cedrene had energy (-5.3 and -5.7 Kcal/mol) for targeted protein of Aspergillus oryzae and (-6.8 and -8.0 Kcal/mol) for Saccharomyces cerevisiae, whereas alpha-bergamotene and Beta-Cedrene exihbit energy (-7.5 and -7.4 Kcal/mol), respectively. The molecular dynamics study confirms the structural stability and rigidity of the docked complex through multiple descriptors from simulation trajectories. The above findings indicated that HbGT6-07 could attain competitive progress via production of VOCs and comprehensive mycelial growth.
Details
- Title
- In vitro and in silico approach of fungal growth inhibition by Trichoderma asperellum HbGT6-07 derived volatile organic compounds
- Creators
- Md Kamaruzzaman - Nanjing Agricultural UniversityMd Samiul Islam - Huazhong Agricultural UniversityShafi Mahmud - University of RajshahiShakil Ahmed Polash - RMIT UniversityRazia Sultana - Huazhong Agricultural UniversityMd Amit Hasan - University of RajshahiChao Wang - Ministry of Ecology and EnvironmentChunhao Jiang - Nanjing Agricultural University
- Publication Details
- Arabian journal of chemistry, Vol.14(9), pp.1-21
- Publisher
- Scientific Scholar; AMSTERDAM
- Grant note
- Financial support for this work came from the National Natural Science Foundation of China (31972322, 31672075, 31701829) , Key research and development projects in Jiangsu Province (BE2020408), Independent innovation project of agricultural science and technology in Jiangsu Province (CX (19)2008), Technical system of Chinese herbal medicine industry (CARS-21), Opening project of key construction laboratory of probiotics in Jiangsu Province (JSYSZJ2019003) and China Postdoctoral Science Foundation (2019 M651863).
- Identifiers
- 991013352972102368
- Copyright
- © The Author(s) 2021.
- Academic Unit
- Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article