Journal article
Identification and characterization of QTL controlling Agrobacterium-mediated transient and stable transformation of Brassica oleracea
Plant Biotechnology Journal, Vol.2(1), pp.59-69
2004
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Abstract
<p>A commonly encountered difficulty with the genetic engineering of crop plants is that different varieties of a particular species can show great variability in the efficiency with which they can be transformed. This increases the effort required to introduce transgenes into particular genetic backgrounds. The use of Substitution Lines has allowed the finer mapping of three Quantitative Trait Loci (<em>tf1</em>, <em>tf2</em> and <em>tf3</em>) that explain 26% of the variation in the efficiency of <em>Agrobacterium</em>-mediated transformation in <em>Brassica oleracea</em>. Use of an ‘orthogonal set’ of genotypes (containing all eight possible combinations of ‘positive’ and ‘negative’ alleles at the three QTL), along with time course studies of transgene expression, has allowed the determination of the stages at which these genes have their effects during transformation. With regard to control of the level of transient transgene expression, <em>tf1</em> (on LGO1) alone has no detectable effect, whilst <em>tf2</em> (on LGO3) and <em>tf3</em> (on LGO7) have highly significant effects (<em>P</em> < 0.001). All three loci have highly significant (<em>P</em> < 0.001) effects on the levels of expression of stably integrated transgene. The use of RFLP markers has shown that <em>tf1</em> and <em>tf2</em> are in duplicated regions of the <em>B. oleracea</em> genome and appear to be paralogous in origin. Colinearity of these regions with the <em>A. thaliana</em> genome has been identified. The results allow the selection of progeny <em>Brassica oleracea</em> genotypes that are more efficiently transformed than either parent used in the original cross.</p>
Details
- Title
- Identification and characterization of QTL controlling Agrobacterium-mediated transient and stable transformation of Brassica oleracea
- Creators
- N OI Cogan - Horticulture Research International, UKH J Newbury - University of Birmingham, UKA M Oldacres - University of Birmingham, UKJ R Lynn - Horticulture Research International, UKM J Kearsey - University of Birmingham, UKGraham J King - Horticulture Research International, UKI J Puddephat - Horticulture Research International, UK
- Publication Details
- Plant Biotechnology Journal, Vol.2(1), pp.59-69
- Identifiers
- 1557; 991012821100102368
- Academic Unit
- Faculty of Science and Engineering; Southern Cross Plant Science; Science
- Resource Type
- Journal article