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Published (Version of record)CC BY V4.0, Open Access
Published (Version of record)CC BY V4.0, Open
Journal article
Histological Dissection of Fusarium-Banana Interaction Using a GFP-Tagged Subtropical Race 4 Strain of Fusarium oxysporum f. sp. cubense on Banana Cultivars with Differing Levels of Resistance
Microorganisms, Vol.12(12), pp.1-21
01/12/2024
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Abstract
Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), poses a significant threat to global banana production. This study used a GFP-tagged subtropical race 4 strain of Foc (GFP-Foc-STR4) to trace the pathogen’s movement in different banana cultivars. These include a race 4 resistant cultivar FHIA25 and the Cavendish somaclone ‘GCTCV119’, as well as susceptible cultivars including ‘Lady Finger’, ‘FHIA02’, and ‘Williams’ Cavendish. GFP localization revealed that GFP-Foc-STR4 was able to infect all tested cultivars, moving from the roots to the rhizome and aerial parts of the plant. Tyloses formation in root and rhizome vasculature, visualised with GFP autofluorescence and confirmed by scanning electron microscopy, was found to restrict Foc within the xylem vessels, slowing its spread but not fully preventing infection. This containment mechanism contributes to the host tolerance of ‘FHIA25’ and ‘GCTCV119’, though it does not confer complete immunity. The use of the fluorescently tagged Foc strain provides valuable insight into the infection process, and supports efforts in the integrated management of Fusarium wilt of banana.
Details
- Title
- Histological Dissection of Fusarium-Banana Interaction Using a GFP-Tagged Subtropical Race 4 Strain of Fusarium oxysporum f. sp. cubense on Banana Cultivars with Differing Levels of Resistance
- Creators
- Andrew Chen - The University of QueenslandChou Ting-Yan - The University of QueenslandYi Chen - The University of QueenslandSumayyah Fallatah - The University of QueenslandJay Anderson - The University of QueenslandJiaman Sun - The University of QueenslandHarry Cosgrove - The University of QueenslandSiyuan Gao - The University of QueenslandBrett Ferguson - The University of QueenslandAmelie Soper - The University of QueenslandDonald Gardiner - The University of QueenslandElizabeth Aitken - The University of Queensland
- Publication Details
- Microorganisms, Vol.12(12), pp.1-21
- Publisher
- MDPI AG; BASEL
- Grant note
- This project was funded by the Bill and Melinda Gates Foundation (Grant ID number: OPP1093845) through its grant to the International Institute of Tropical Agriculture (IITA) under the project Accelerated Breeding of Better Bananas, grant number IITA 20600.15/0008-8—Phase II. Research funding support for this project was also provided by Hort Innovation Australia under grant BA17006.
- Identifiers
- 991013245546502368
- Copyright
- © 2024 by the authors.
- Academic Unit
- Faculty of Science and Engineering; Science
- Language
- English
- Resource Type
- Journal article