High throughput screening of fungal phytopathogens caught in Australian forestry insect surveillance traps

Conrad Trollip; Angus J. Carnegie; Alexander M. Piper; Jatinder Kaur; Francesco Martoni; Quang Dinh; David Smith; Ross Mann; Brendan Rodoni; Jacqueline Edwards

doi:10.3389/ffgc.2023.1149755

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High throughput screening of fungal phytopathogens caught in Australian forestry insect surveillance traps

Journal article

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High throughput screening of fungal phytopathogens caught in Australian forestry insect surveillance traps

Conrad Trollip, Angus J. Carnegie, Alexander M. Piper, Jatinder Kaur, Francesco Martoni, Quang Dinh, David Smith, Ross Mann, Brendan Rodoni and Jacqueline Edwards

Frontiers in Forests and Global Change, Vol.6, 1149755

28/03/2023

DOI: https://doi.org/10.3389/ffgc.2023.1149755

Appears in Recent Faculty of Science and Engineering Publications

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Abstract

forest biosecurity

environmental DNA sequencing

metabarcoding

fungal diagnostics

bark beetles

Ophiostomatales

Post-border surveillance for forestry's high priority pests and pathogens is conducted routinely through established programs focused on the main points-of-entry and across the major plantation growing regions. Currently, most diagnostic protocols used to identify fungal phytopathogens sampled during these surveys rely on traditional methods, such as morphological examination and DNA barcoding techniques. This stepwise process from isolation to species identification is often regarded as slow, expensive, and limited due to the need for disease manifestation and/or comprehensive expertise for rapid and accurate detection. In this study, we applied a recently validated high-throughput, dual-marker amplicon sequencing approach on insect surveillance traps from across Australia to assess its performance for the targeted surveillance of the Ophiostomatales, an order of fungi comprising notable phytopathogens which are vectored by bark beetles. By using a recently validated assay we were able to confidently characterize a range of Ophiostomatalean taxa known to be present in Australia, while reporting eight first detections from environmental DNA. Our study demonstrates the value of targeted multi-barcode amplicon sequencing for high-throughput screening of fungi caught in post-border surveillance traps, in addition to emphasizing research priorities that require further investigation before such methods can be implemented routinely for biosecurity.

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Title: High throughput screening of fungal phytopathogens caught in Australian forestry insect surveillance traps
Creators: Conrad Trollip - La Trobe University
Angus J. Carnegie - NSW Department of Primary Industries
Alexander M. Piper - Department of Energy, Environment and Climate Action (Bundoora, Australia)
Jatinder Kaur - Department of Energy, Environment and Climate Action (Bundoora, Australia)
Francesco Martoni - Department of Energy, Environment and Climate Action (Bundoora, Australia)
Quang Dinh - Department of Energy, Environment and Climate Action (Bundoora, Australia)
David Smith - Murdoch University
Ross Mann - Department of Energy, Environment and Climate Action (Bundoora, Australia)
Brendan Rodoni - La Trobe University
Jacqueline Edwards - La Trobe University
Publication Details: Frontiers in Forests and Global Change, Vol.6, 1149755
Publisher: Frontiers Media Sa
Number of pages: 14
Grant note: ST16010 / Australian Government Department of Agriculture; Australian Government La Trobe University Forest and Wood Products Australia La Trobe University Full Fee Research Scholarship at La Trobe University, Victoria, Australia
Identifiers: 991013176692002368
Copyright: © 2023 Trollip, Carnegie, Piper, Kaur, Martoni, Dinh, Smith, Mann, Rodoni and Edwards. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Academic Unit: Faculty of Science and Engineering
Language: English
Resource Type: Journal article

High throughput screening of fungal phytopathogens caught in Australian forestry insect surveillance traps

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