Journal article
Genome-wide association study dissects the genetic control of plant height and branch number in response to low-phosphorus stress in Brassica napus
Annals of Botany, Vol.128(7), pp.919-930
09/11/2021
PMCID: PMC8577194
PMID: 34490877
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Abstract
Background and Aims: Oilseed rape (Brassica napus) is one of the most important oil crops worldwide. Phosphorus (P) deficiency severely decreases the plant height and branch number of B. napus. However, the genetic bases controlling plant height and branch number in B. napus under P deficiency remain largely unknown. This study aims to mine candidate genes for plant height and branch number by genome-wide association study (GWAS) and determine low-P-tolerance haplotypes.
Methods: An association panel of B. napus was grown in the field with a low P supply (P, 0 kg ha−1) and a sufficient P supply (P, 40 kg ha−1) across 2 years and plant height and branch number were investigated. More than five million single-nucleotide polymorphisms (SNPs) were used to conduct GWAS of plant height and branch number at two contrasting P supplies.
Key Results: A total of 2127 SNPs were strongly associated (P < 6·25 × 10−07) with plant height and branch number at two P supplies. There was significant correlation between phenotypic variation and the number of favourable alleles of associated loci on chromosomes A10 (chrA10_821671) and C08 (chrC08_27999846), which will contribute to breeding improvement by aggregating these SNPs. BnaA10g09290D and BnaC08g26640D were identified to be associated with chrA10_821671 and chrC08_27999846, respectively. Candidate gene association analysis and haplotype analysis showed that the inbred lines carrying ATT at BnaA10g09290Hap1 and AAT at BnaC08g26640Hap1 had greater plant height than lines carrying other haplotype alleles at low P supply.
Conclusion: Our results demonstrate the power of GWAS in identifying genes of interest in B. napus and provided insights into the genetic basis of plant height and branch number at low P supply in B. napus. Candidate genes and favourable haplotypes may facilitate marker-based breeding efforts aimed at improving P use efficiency in B. napus.
Details
- Title
- Genome-wide association study dissects the genetic control of plant height and branch number in response to low-phosphorus stress in Brassica napus
- Creators
- Haijiang Liu - Huazhong Agricultural UniversityJingchi Wang - Huazhong Agricultural UniversityBingbing Zhang - Huazhong Agricultural UniversityXinyu Yang - Huazhong Agricultural UniversityJohn P Hammond - University of ReadingGuangda Ding - Huazhong Agricultural UniversitySheliang Wang - Huazhong Agricultural UniversityHongmei Cai - Huazhong Agricultural UniversityChuang Wang - Huazhong Agricultural UniversityFangsen Xu - Huazhong Agricultural UniversityLei Shi - Huazhong Agricultural University
- Publication Details
- Annals of Botany, Vol.128(7), pp.919-930
- Publisher
- Oxford University Press
- Grant note
- This work was supported by the National Nature Science Foundation of China (Grant No. 31972498). We also acknowledge the Applied Basic Research Fronts Program of Wuhan city (Grant No. 2018020401011302), the National Key R & D Program of China (Grant No. 2017YFD0200200) and the Natural and Fundamental Research Funds for the Central Universities of China (Grant No. 2662019PY013).
- Identifiers
- 991012982988102368
- Copyright
- © The Author(s) 2021. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
- Academic Unit
- School of Environment, Science and Engineering; Science; Southern Cross Plant Science; Faculty of Science and Engineering
- Language
- English
- Resource Type
- Journal article