Journal article
Functional homoeologous alleles of CONSTANS contribute to seasonal crop type in rapeseed
Theoretical and Applied Genetics: international journal of plant breeding researc, Vol.134(10), pp.3287-3303
10/2021
PMID: 34410456
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Source: InCites
Abstract
Key message
Two CO paralogs in Brassica napus were confirmed and shown distinct expression pattern and function in promoting flowering and allelic variation s within BnaCO.A10 were found closely associated with ecotype divergence.
Abstract
CONSTANS (CO) is a key gene that responds to photoperiod and in Arabidopsis can promote flowering under long-day (LD) conditions. Brassica napus L. is a major oil crop and close relative of Arabidopsis, and arose via allopolyploidization from the diploids B. rapa (A genome) and B. oleracea (C genome). In this study, we confirmed that B. napus has two CO genes located on the A10 (BnaCO.A10) and C9 (BnaCO.C9) chromosomes. Significant differences in level and temporal pattern of transcription, as well as in protein function, of these homoeologous may have resulted from sequence variation in the promoter as well as in the coding region. Apart from two insertions of 527 bp and 2002 bp in the promoter of BnaCO.C9 that function as transcriptional enhancers, this gene is otherwise highly conserved in both promoter and coding region. However, BnaCO.A10 was classified into two haplotypes and transgene analysis in Arabidopsis and backcross analysis in rapeseed indicated that the winter-type haplotype had a greater effect in promoting flowering than the spring type. We discuss the contribution of CO alleles to species evolution, and for eco-geographic radiation following crop domestication, alongside scope for managing this locus in future breeding.
Details
- Title
- Functional homoeologous alleles of CONSTANS contribute to seasonal crop type in rapeseed
- Creators
- Qingdong Jin - Huazhong Agricultural UniversityShuai Yin - Huazhong Agricultural UniversityGe Li - Huazhong Agricultural UniversityTao Guo - Huazhong Agricultural UniversityMing Wan - Huazhong Agricultural UniversityHaitao Li - Huazhong Agricultural UniversityJuanjuan Li - National Key Lab of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural UniversityXianhong Ge - Huazhong Agricultural UniversityGraham J King - Southern Cross UniversityZaiyun Li - Huazhong Agricultural UniversityJing Wang - Huazhong Agricultural UniversityGuangsheng Zhou - Huazhong Agricultural University
- Publication Details
- Theoretical and Applied Genetics: international journal of plant breeding researc, Vol.134(10), pp.3287-3303
- Publisher
- Springer
- Grant note
- This work was supported by the National Key Research and Development Program of China (2017YFD0101700), the Fundamental Research Funds for the Central Universities (2662020ZKPY021), National Key Research and Development Program of China (2018YFD1000900) and the National Natural Science Foundation of China (31,471,531).
- Identifiers
- 991012961198302368
- Copyright
- © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021
- Academic Unit
- Faculty of Science and Engineering; School of Environment, Science and Engineering; Science; Southern Cross Plant Science
- Language
- English
- Resource Type
- Journal article