Journal article
Fine mapping of stripe rust resistance gene YrAn1589 in common wheat using Wheat660K SNP array and BSR‑Seq
Theoretical and applied genetics, Vol.138, 63
03/2025
PMID: 40021553
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Abstract
Stripe rust, caused by Puccinia. striiformis f. sp. tritici (Pst), is a devastating fungal disease that can significantly reduce wheat yield. The Chinese wheat cultivar Annong1589 demonstrates high resistance against the predominant Pst races in the Huang-Huai valley wheat region. The present study aimed to identify the stripe rust resistance gene in Annong1589. Genetic analysis indicated that the resistance in Annong1589 was conferred by a single dominant gene, provisionally designated YrAn1589. Using Wheat660K SNP array, bulked segregant RNA sequencing and new molecular markers developed, the resistance gene was mapped to a 160.9–166.6 kb region between CAPS8 and CAPS10 on chromosome 3BL based on IWGSC CS RefSeq v2.1 and eight other reference genome sequences, including eight high-confidence annotated genes. Transcriptome and qRT-PCR analyses revealed significantly upregulated expression of TraesCS3B03G1054600 in resistant plants following CYR32 inoculation, suggesting it is a potential candidate gene for YrAn1589. A functional marker CAPS9 developed from a A/G polymorphic SNP in the candidate co-segregated with YrAn1589 in the F2 population. Subcellular localization experiments showed that TraesCS3B03G1054600 protein was localized in the cytoplasm and nucleus, implying its role in immune response and resistance. Our findings establish YrAn1589 as a new stripe rust resistance gene, providing valuable gene resource and molecular markers for improvement of stripe rust resistance in wheat.
Details
- Title
- Fine mapping of stripe rust resistance gene YrAn1589 in common wheat using Wheat660K SNP array and BSR‑Seq
- Creators
- Weihao Hao - Anhui Agricultural UniversityYingjie Wu - Anhui Agricultural UniversityQi Guo - Southern Cross University, Research Infrastructure and OperationsJingchun Wu - Shandong Agricultural UniversityMeng Lin - Anhui Agricultural University (China)Qiwei Hu - Anhui Agricultural University (China)Erwin Tandayu - Agriculture Victoria Services (Australia)Jie Lu - Anhui Agricultural University (China)Hongqi Si - Ministry of Agriculture and Rural AffairsChuanxi Ma - Ministry of Agriculture and Rural AffairsXiaobo Wang - Anhui Agricultural UniversityCan Chen - Ministry of Agriculture and Rural Affairs
- Publication Details
- Theoretical and applied genetics, Vol.138, 63
- Publisher
- Springer Nature
- Grant note
- This work was funded by the grants from the Natural Science Research Project of Anhui Educational Committee (2022AH050887), the Agriculture Research System (CARS-03), the key scientific and technological breakthroughs in Anhui Province (2021d06050003), Anhui Provincial and Ministerial Collaborative Innovation Center for Food Crops (APMCIC-FC), and Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP).
- Identifiers
- 991013265311002368
- Copyright
- © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025
- Academic Unit
- Research Infrastructure and Operations
- Language
- English
- Resource Type
- Journal article