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Journal article
Enteric Viruses and Free-Living Amoebae: Protozoa as Potential Reservoirs and Transport Vessels for Human Norovirus and Adenovirus
Water & Ecology, Vol.First online, pp.1-34
22/09/2025
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Abstract
Human norovirus (HNoV) and human adenovirus (HAdV) are major causes of acute viral gastroenteritis globally and environmentally transmitted via the faecal-oral route through contaminated food and water. Recent evidence of these enteric viruses residing within environmental free-living amoebae (FLA)—specifically Vermamoeba vermiformis, Acanthamoeba polyphaga, and Willaertia magna, all abundant in natural and engineered water systems—has significant implications for environmental virology and public health. The incorporation of HNoV into the cytoplasm and vacuoles of Vermamoeba vermiformis and Acanthamoeba polyphaga, as well as the nuclear localization of HAdV within Willaertia magna, was demonstrated using quantitative polymerase chain reaction (qPCR) and fluorescence microscopy. Intact HNoV and HAdV virions persisted inside FLA trophozoites, cysts, and extracellular vesicles for up to 12 days. Moreover, HAdV retained infectivity in buffalo green monkey kidney cells following intracellular persistence, suggesting these viruses can evade amoebal digestion and structural degradation. In the case of HAdV, nuclear incorporation, preservation of capsid integrity, and detection of messenger RNA associated with adenoviral fiber protein synthesis further suggest the possible initiation of virus-related transcriptional activity within the amoeba host. These findings challenge current assumptions about virus removal rates in sewage treatment, food safety protocols, and drinking water production. The enhanced persistence and protection conferred by ubiquitous FLA may also impact microbial risk assessments for recreational water use, particularly in sewage-impacted rivers and lakes, as well as for water reuse applications. Recognition of FLA as reservoirs and transport vessels for enteric viruses necessitates a re-evaluation of existing water and sanitation guidelines to better mitigate environmental transmission pathways.
Details
- Title
- Enteric Viruses and Free-Living Amoebae: Protozoa as Potential Reservoirs and Transport Vessels for Human Norovirus and Adenovirus
- Creators
- Mats Leifels - Karl Landsteiner University of Health SciencesRafik Dey - University of AlbertaAlyssa Wiedmann - University of AlbertaCheng Dan - Nanyang Technological UniversityClaudia Kolm - Karl Landsteiner University of Health SciencesFuqing Wu - The University of Texas Health Science Center at HoustonKwanrawee Sirikanchana - Chulabhorn Research InstituteAndreas H Farnleitner - Karl Landsteiner University of Health SciencesNicholas J Ashbolt - Adelaide University
- Publication Details
- Water & Ecology, Vol.First online, pp.1-34
- Publisher
- Elsevier B.V.
- Grant note
- This research was supported by the Singapore National Research Foundation and Ministry of Education under the Research Centre of Excellence Program, and the Canada Foundation for Innovation, CHIR, Alberta Innovates. Part of the study was funded by the Gateway Fellowship of the RUB Research School, Ruhr-University Bochum, Germany
- Identifiers
- 991013317528102368
- Copyright
- © 2025 The Authors.
- Academic Unit
- Faculty of Science and Engineering; Science
- Language
- English
- Resource Type
- Journal article